C57BL/6JCya-Klc2em1/Cya
Common Name
Klc2-KO
Product ID
S-KO-19491
Backgroud
C57BL/6JCya
Strain ID
KOCMP-16594-Klc2-B6J-VA
Status
When using this mouse strain in a publication, please cite “Klc2-KO Mouse (Catalog S-KO-19491) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Klc2-KO
Strain ID
KOCMP-16594-Klc2-B6J-VA
Gene Name
Product ID
S-KO-19491
Gene Alias
KLC 2, KLC-2, 8030455F02Rik
Background
C57BL/6JCya
Gene Full Name
kinesin light chain 2
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 19 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000116563
NCBI Transcript ID
NM_008451
Target Region
Exon 3~4
Size of Effective Region
~1.3 kb
Overview of Gene Research
Klc2, the light chain of kinesin-1, mediates cargo binding and regulates kinesin-1 motility, which is crucial for the microtubule-based intracellular transport system [3]. It is involved in various cellular processes related to the movement of vesicles and organelles along microtubules. The study of Klc2 in genetic models, especially KO mouse models, has been valuable for understanding its functions.
In Klc2-deficient C57BL/6 J mice, low-frequency sensorineural hearing loss occurs as early as 1 month of age. This deficiency leads to abnormal mitochondrial transport and down-regulation of the GABAA receptor family, indicating Klc2's essential role in cochlear hair cell function [1].
In zebrafish (Danio rerio), knockdown and overexpression of klc2 result in a mild to severe curly-tail phenotype, suggesting its role in neuromuscular disorders [2].
In non-small cell lung cancer (NSCLC), down-regulation of KLC2 can increase radiosensitivity by activating the P53 pathway, while KLC2 forms a positive feedback loop with HuR to promote radioresistance [4].
In conclusion, Klc2 is essential for functions like cochlear hair cell function, potentially in neuromuscular processes, and in influencing radiosensitivity in NSCLC. Studies using KO mouse models and other genetic models have been instrumental in revealing Klc2's functions in these specific disease-related biological processes, highlighting its importance in understanding sensorineural hearing loss, neuromuscular disorders, and NSCLC radiotherapy.
References：
1. Fu, Xiaolong, An, Yachun, Wang, Hongyang, Gao, Jiangang, Chai, Renjie. 2021. Deficiency of Klc2 Induces Low-Frequency Sensorineural Hearing Loss in C57BL/6 J Mice and Human. In Molecular neurobiology, 58, 4376-4391. doi:10.1007/s12035-021-02422-w. https://pubmed.ncbi.nlm.nih.gov/34014435/
2. Melo, Uirá S, Macedo-Souza, Lucia I, Figueiredo, Thalita, Zatz, Mayana, Santos, Silvana. 2015. Overexpression of KLC2 due to a homozygous deletion in the non-coding region causes SPOAN syndrome. In Human molecular genetics, 24, 6877-85. doi:10.1093/hmg/ddv388. https://pubmed.ncbi.nlm.nih.gov/26385635/
3. Cockburn, Joseph J B, Hesketh, Sophie J, Mulhair, Peter, O'Connell, Mary J, Way, Michael. 2018. Insights into Kinesin-1 Activation from the Crystal Structure of KLC2 Bound to JIP3. In Structure (London, England : 1993), 26, 1486-1498.e6. doi:10.1016/j.str.2018.07.011. https://pubmed.ncbi.nlm.nih.gov/30197037/
4. Qiao, Simiao, Jiang, Yuhang, Li, Na, Zhu, Xiaoxia. 2023. The kinesin light chain-2, a target of mRNA stabilizing protein HuR, inhibits p53 protein phosphorylation to promote radioresistance in NSCLC. In Thoracic cancer, 14, 1440-1450. doi:10.1111/1759-7714.14886. https://pubmed.ncbi.nlm.nih.gov/37055376/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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