C57BL/6JCya-Fam20aem1/Cya
Common Name
Fam20a-KO
Product ID
S-KO-20225
Backgroud
C57BL/6JCya
Strain ID
KOCMP-208659-Fam20a-B6J-VB
Status
When using this mouse strain in a publication, please cite “Fam20a-KO Mouse (Catalog S-KO-20225) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Fam20a-KO
Strain ID
KOCMP-208659-Fam20a-B6J-VB
Gene Name
Product ID
S-KO-20225
Gene Alias
--
Background
C57BL/6JCya
Gene Full Name
FAM20A, golgi associated secretory pathway pseudokinase
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000020938
NCBI Transcript ID
NM_153782
Target Region
Exon 5~6
Size of Effective Region
~1.2 kb
Overview of Gene Research
Fam20a, Family with Sequence Similarity 20, Member A, is a pseudo-kinase. It binds to FAM20C (the Golgi casein kinase) and potentiates its function to phosphorylate secreted proteins crucial for biomineralization. This function is vital for processes like amelogenesis and maintaining pulp tissue homeostasis, preventing ectopic mineralization in soft tissues [1].
Gene knockout mouse models have provided valuable insights. In Sox2-Cre;Fam20afl/fl mice, Fam20a ablation in both dental epithelium and mesenchyme led to an enamel phenotype resembling human amelogenesis imperfecta associated with FAM20A mutations, but no apparent dentin defects. Secretory-stage ameloblasts were abnormal, and enamel-matrix-protein-encoding genes were downregulated [2]. Epithelial-specific deletion of Fam20a in mice caused short root defects, with irregular breaks in the epithelial root sheath, initiation of the WNT signaling pathway, and decreased expression of Cdc42. It also affected the intraosseous eruption phase of tooth emergence, delaying osteoclast peak and tooth eruption [3].
In conclusion, Fam20a is essential for amelogenesis and tooth root development, playing a key role in maintaining the normal structure and function of dental tissues. The study of Fam20a using gene knockout mouse models has enhanced our understanding of its functions in these processes and its association with diseases such as enamel-renal syndrome, amelogenesis imperfecta, and short root defects [1,2,3].
References：
1. Wang, Shih-Kai, Zhang, Hong, Wang, Yin-Lin, Simmer, James P, Hu, Jan C-C. 2023. FAM20A mutations and transcriptome analyses of dental pulp tissues of enamel renal syndrome. In International endodontic journal, 56, 943-954. doi:10.1111/iej.13928. https://pubmed.ncbi.nlm.nih.gov/37159186/
2. Li, Lili, Saiyin, Wuliji, Zhang, Hua, Qin, Chunlin, Lu, Yongbo. 2019. FAM20A is essential for amelogenesis, but is dispensable for dentinogenesis. In Journal of molecular histology, 50, 581-591. doi:10.1007/s10735-019-09851-x. https://pubmed.ncbi.nlm.nih.gov/31667691/
3. Liu, Lixue, Yao, Lihong, Lu, Zeyu, Jin, Han, Zhang, Bin. 2023. Epithelial-specific deletion of FAM20A leads to short root defects. In Gene, 884, 147731. doi:10.1016/j.gene.2023.147731. https://pubmed.ncbi.nlm.nih.gov/37625561/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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