C57BL/6JCya-Arl6ip5em1/Cya
Common Name
Arl6ip5-KO
Product ID
S-KO-20235
Backgroud
C57BL/6JCya
Strain ID
KOCMP-65106-Arl6ip5-B6J-VA
When using this mouse strain in a publication, please cite “Arl6ip5-KO Mouse (Catalog S-KO-20235) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Arl6ip5-KO
Strain ID
KOCMP-65106-Arl6ip5-B6J-VA
Gene Name
Product ID
S-KO-20235
Gene Alias
5930404D22Rik, Aip-5, Gtrap3-18, addiscin
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000044681
NCBI RefSeq
NM_022992
Target Region
Exon 2
Size of Effective Region
~2.3 kb
Overview of Gene Research
Arl6ip5, also known as JWA, is an endoplasmic reticulum-localized protein belonging to the prenylated rab-acceptor-family. It is involved in multiple biological processes such as exocytic protein trafficking, glutathione metabolism, and has been implicated in various pathways related to cell survival, autophagy, and apoptosis. Its study through genetic models is crucial for understanding its functions [3,5].
In ovarian carcinoma, overexpression of ARL6IP5 reduces cisplatin-resistance by suppressing DNA repair (XRCC1 and PARP1) and promoting apoptosis, acting as a tumor-suppressor [1]. In Parkinson's disease models, reduced ARL6IP5 levels are observed. Overexpressing ARL6IP5 ameliorates α-synuclein burden by inducing autophagy via preventing ubiquitination and degradation of ATG12, and JWA (ARL6IP5) alleviates dopaminergic neuron degeneration by suppressing ferritinophagy [2,4]. In bone-related studies, Arl6ip5 knockout mice show decreased bone mineral density. Osteoblastic Arl6ip5 deficiency impairs osteoblast differentiation and enhances osteoclastogenesis through ER calcium homeostasis disturbance and ER stress-mediated apoptosis, indicating its role as a regulator of bone formation [5].
In conclusion, ARL6IP5 plays essential roles in multiple biological processes including cancer development, neurodegeneration, and bone metabolism. Gene knockout models in mice have been instrumental in revealing its functions in these disease-related areas, helping to understand the underlying mechanisms and potentially paving the way for new therapeutic strategies.
References：
1. Kim, Ji-Ye, Bahar, Entaz, Lee, Jung-Yun, Yoon, Hyonok, Kim, Hyun-Soo. 2022. ARL6IP5 reduces cisplatin-resistance by suppressing DNA repair and promoting apoptosis pathways in ovarian carcinoma. In Cell death & disease, 13, 239. doi:10.1038/s41419-022-04568-4. https://pubmed.ncbi.nlm.nih.gov/35293383/
2. Siddique, Ibrar, Kamble, Kajal, Gupta, Sakshi, Ahsan, Nuzhat, Gupta, Sarika. 2023. ARL6IP5 Ameliorates α-Synuclein Burden by Inducing Autophagy via Preventing Ubiquitination and Degradation of ATG12. In International journal of molecular sciences, 24, . doi:10.3390/ijms241310499. https://pubmed.ncbi.nlm.nih.gov/37445677/
3. Wu, Yu, Wang, Miaomiao, Peng, Ying, Deng, Lili, Fu, Qiang. 2015. Overexpression of Arl6ip5 in osteoblast regulates RANKL subcellualr localization. In Biochemical and biophysical research communications, 464, 1275-1281. doi:10.1016/j.bbrc.2015.07.119. https://pubmed.ncbi.nlm.nih.gov/26220341/
4. Zhao, Xinxin, Kang, Zhengwei, Han, Ruixue, Cao, Lei, Lu, Ming. 2024. JWA binding to NCOA4 alleviates degeneration in dopaminergic neurons through suppression of ferritinophagy in Parkinson's disease. In Redox biology, 73, 103190. doi:10.1016/j.redox.2024.103190. https://pubmed.ncbi.nlm.nih.gov/38744191/
5. Wu, Y, Yang, M, Fan, J, Miao, D, Fu, Q. 2014. Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis. In Cell death & disease, 5, e1464. doi:10.1038/cddis.2014.427. https://pubmed.ncbi.nlm.nih.gov/25321471/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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