C57BL/6JCya-Larp7em1/Cya
Common Name:
Larp7-KO
Product ID:
S-KO-20374
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Larp7-KO
Strain ID
KOCMP-28036-Larp7-B6J-VB
Gene Name
Product ID
S-KO-20374
Gene Alias
C330027G06Rik; D3Wsu161e
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Larp7em1/Cya mice (Catalog S-KO-20374) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029588
NCBI RefSeq
NM_138593
Target Region
Exon 2~3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Larp7, short for La ribonucleoprotein domain family member 7, is a master regulator involved in multiple essential biological functions. It governs the DNA damage response and RNAPII pausing pathway. Larp7 binds to the 7SK RNA as part of a 7SK small nuclear ribonucleoprotein, which inhibits the transcriptional activity of RNA polymerase II. It also plays roles in the assembly of other RNPs, as well as in the modification, processing and cellular transport of RNA molecules [3].
Global and cardiac-specific Larp7 knockout (KO) mouse models have been crucial in understanding its function. Constitutive Larp7 KO in mice leads to impaired mitochondrial biogenesis, myocardial hypoplasia, and mid-gestational lethality. Cardiac-specific inactivation results in defective mitochondrial biogenesis, impaired oxidative phosphorylation, elevated oxidative stress, and heart failure by 4 months of age. These effects are accompanied by reduced SIRT1 stability and deacetylase activity, which impairs SIRT1-mediated transcription of genes for oxidative phosphorylation and energy metabolism, dampening cardiac function. In a mouse model of myocardial infarction, restoring Larp7 expression improves the function of the injured heart. Also, deletion of Larp7 in a rodent model leads to senescent cell accumulation and premature aging, accelerating cellular senescence [1,2].
In conclusion, Larp7 is essential for mitochondrial biogenesis, energy production, and cardiac function by modulating SIRT1 homeostasis and activity. Mouse KO models have revealed its significance in heart failure pathogenesis. Additionally, Larp7 plays a role in preventing cellular senescence and aging. Understanding Larp7's functions through these models provides potential therapeutic targets for heart-related diseases and age-associated conditions.
References:
1. Yu, Huijing, Zhang, Fang, Yan, Pengyi, Sun, Kun, Zhang, Bing. 2021. LARP7 Protects Against Heart Failure by Enhancing Mitochondrial Biogenesis. In Circulation, 143, 2007-2022. doi:10.1161/CIRCULATIONAHA.120.050812. https://pubmed.ncbi.nlm.nih.gov/33663221/
2. Yan, Pengyi, Li, Zixuan, Xiong, Junhao, Huang, Yu, Zhang, Bing. . LARP7 ameliorates cellular senescence and aging by allosterically enhancing SIRT1 deacetylase activity. In Cell reports, 37, 110038. doi:10.1016/j.celrep.2021.110038. https://pubmed.ncbi.nlm.nih.gov/34818543/
3. Hasler, Daniele, Meister, Gunter, Fischer, Utz. 2020. Stabilize and connect: the role of LARP7 in nuclear non-coding RNA metabolism. In RNA biology, 18, 290-303. doi:10.1080/15476286.2020.1767952. https://pubmed.ncbi.nlm.nih.gov/32401147/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen