The TAG stop codon was replaced with "P2A-CreERT2" cassette. CreERT2 recombinase is expressed under the regulatory control of Ace2 gene elements.

The TAA stop codon was replaced with the "P2A-CreERT2" cassette. CreERT2 recombinase is expressed under the regulatory control of Adgre1 gene elements. This model is a Tamoxifen-inducible Cre mouse, and when crossed with mice containing loxP sites, the offspring mice are expected to undergo sequence recombination between loxP sites mediated by Cre recombinase in macrophages following Tamoxifen induction.

The “Cre-P2A” cassette will be inserted upstream of the ATG start codon.

The TAG stop codon was replaced with "3xGGGGS-EGFP" cassette. Enhanced green fluorescent protein (EGFP) is expressed under the regulatory control of Adgrg3 gene elements, without impacting the endogenous expression of the Adgrg3 gene. This model enables EGFP fluorescence protein tracing studies.

Adgrl2-3xGGGGS-mCherry mice are constructed by replacing the partial exon 1 coding region of the mouse Adgrl2 gene with HA signal peptide - HA tag - Mouse Adgrl2 CDS (without signal peptide) - 3xGGGGS - mCherry - rBG pA cassette using gene editing technology. The Adgrl2-3xGGGGS-mCherry mouse carries a red fluorescent protein (mCherry) expression cassette, making it a precise research model that maintains protein function while offering fluorescence visualization. This model is valuable for several key areas of study. For instance, it can be used for the spatio-temporal dynamic analysis of the Adgrl2 gene expression profile. Researchers can also utilize it for investigating neuronal development and synapse formation mechanisms. Furthermore, it enables live-animal dynamic tracking and real-time imaging observation, providing invaluable insights. Lastly, this model is well-suited for systematic studies of protein interaction networks and downstream signaling pathways.

The ADIPOQ gene-encoded adiponectin is a protein hormone produced exclusively by adipocytes (fat cells). It is transported through the bloodstream to muscle and liver cells. In this model, the P2A-iCre gene expression element is inserted into the mouse endogenous Adipoq gene at the stop codon. Its specific expression is regulated by the mouse Adipoq gene. When this strain is crossed with mice containing loxP sites, sequence recombination mediated by the Cre recombinase between loxP sites can occur in the white adipose tissue (WAT) and brown adipose tissue (BAT) of its offspring.

The AGRP gene encodes Agouti-related protein (AgRP), a neuropeptide produced by AgRP/NPY neurons in the brain, primarily expressed in the arcuate nucleus of the hypothalamus, kidneys, and adrenal glands. Studies have shown that AGRP gene expression significantly increases during starvation and rapidly decreases after feeding. AgRP is synthesized exclusively in the ventromedial part of the arcuate nucleus in cells containing neuropeptide Y (NPY) and is co-expressed with NPY, playing a role in increasing appetite, reducing metabolism, and decreasing energy expenditure. The Agrp-IRES-CreERT2-P2A-tdTomato mouse model was created by integrating the IRES-CreERT2-P2A-tdTomato gene expression cassette into the mouse Agrp gene. Under the control of the mouse endogenous Agrp gene regulatory elements, this mouse expresses tamoxifen-inducible CreERT2 recombinase. Additionally, the expression cassette includes a red fluorescent protein (tdTomato) expression element for lineage tracing of Agrp-positive cells. When Agrp-IRES-CreERT2-P2A-tdTomato mice are crossed with mice containing loxP sites, tamoxifen induction can trigger Cre recombinase-mediated sequence recombination between loxP sites in AgRP-positive neurons of the offspring.

The TGA stop codon was replaced with "P2A-CreERT2" cassette.

The TAA stop codon was replaced with the "P2A-EGFP-T2A-iCre" cassette. Cre recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of Alas2 gene elements, without impacting the endogenous expression of the Alas2 gene. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies.

The TGA stop codon was replaced by "P2A-Cre". A synonymous mutation p.R894=(CGG to CGC) and an additional mutation c.*3C>G in 3'UTR were also introduced to prevent the binding and re-cutting of the sequence by gRNA after homology-directed repair.