C57BL/6JCya-Minpp1em1flox/Cya
Common Name
Minpp1-flox
Product ID
S-CKO-03733
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-17330-Minpp1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Minpp1-flox Mouse (Catalog S-CKO-03733) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Minpp1-flox
Strain ID
CKOCMP-17330-Minpp1-B6J-VA
Gene Name
Product ID
S-CKO-03733
Gene Alias
--
Background
C57BL/6JCya
Gene Full Name
multiple inositol polyphosphate histidine phosphatase 1
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 19 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000025827
NCBI Transcript ID
NM_010799
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Overview of Gene Research
MINPP1, the multiple inositol-polyphosphate phosphatase 1 gene, is crucial for regulating inositol polyphosphate metabolism. Inositol polyphosphates are key metabolic and secondary messengers involved in diverse cellular functions like calcium homeostasis, cell survival, and death [1,3,4,5,6,7]. MINPP1, an endoplasmic reticulum (ER)-resident enzyme, hydrolyzes inositol phosphates, maintaining a proper balance of these molecules in the cell [2,3,5,6,7].
In Minpp1-deficient mice, levels of inositol 1,3,4,5,6-pentakisphosphate (InsP(5)) and inositol hexakisphosphate (InsP(6)) were 30-45% higher than in wild-type cells, indicating that ER-based Minpp1 is important for maintaining the steady-state levels of these polyphosphates [6]. In human patients, loss-of-function mutations in MINPP1 cause a distinct type of Pontocerebellar Hypoplasia, associated with an intracellular imbalance of inositol polyphosphate metabolism, specifically an accumulation of inositol hexakisphosphate (IP6) [1,2]. Patient-derived and genome-edited MINPP1-/-induced stem cells show inefficient neuronal differentiation and increased cell death [1].
In conclusion, MINPP1 plays a vital role in regulating inositol polyphosphate metabolism, which is essential for normal cell growth and brain development. The study of Minpp1-deficient mouse models and human patients with MINPP1 mutations has provided insights into its function in maintaining cellular homeostasis and its role in Pontocerebellar Hypoplasia.
References：
1. Ucuncu, Ekin, Rajamani, Karthyayani, Wilson, Miranda S C, Saiardi, Adolfo, Cantagrel, Vincent. 2020. MINPP1 prevents intracellular accumulation of the chelator inositol hexakisphosphate and is mutated in Pontocerebellar Hypoplasia. In Nature communications, 11, 6087. doi:10.1038/s41467-020-19919-y. https://pubmed.ncbi.nlm.nih.gov/33257696/
2. Appelhof, Bart, Wagner, Matias, Hoefele, Julia, Wieczorek, Dagmar, Jamra, Rami Abou. 2020. Pontocerebellar hypoplasia due to bi-allelic variants in MINPP1. In European journal of human genetics : EJHG, 29, 411-421. doi:10.1038/s41431-020-00749-x. https://pubmed.ncbi.nlm.nih.gov/33168985/
3. Zubair, Mohd, Hamzah, Rabab, Griffin, Robert, Ali, Nawab. 2022. Identification and functional characterization of multiple inositol polyphosphate phosphatase1 (Minpp1) isoform-2 in exosomes with potential to modulate tumor microenvironment. In PloS one, 17, e0264451. doi:10.1371/journal.pone.0264451. https://pubmed.ncbi.nlm.nih.gov/35235602/
4. Nguyen Trung, Minh, Kieninger, Stefanie, Fandi, Zeinab, Keller, Bettina, Fiedler, Dorothea. 2022. Stable Isotopomers of myo-Inositol Uncover a Complex MINPP1-Dependent Inositol Phosphate Network. In ACS central science, 8, 1683-1694. doi:10.1021/acscentsci.2c01032. https://pubmed.ncbi.nlm.nih.gov/36589890/
5. Yu, Jia, Leibiger, Barbara, Yang, Shao-Nian, Berggren, Per-Olof, Barker, Christopher J. 2023. Multiple Inositol Polyphosphate Phosphatase Compartmentalization Separates Inositol Phosphate Metabolism from Inositol Lipid Signaling. In Biomolecules, 13, . doi:10.3390/biom13060885. https://pubmed.ncbi.nlm.nih.gov/37371464/
6. Chi, H, Yang, X, Kingsley, P D, Shears, S B, Reynolds, P R. . Targeted deletion of Minpp1 provides new insight into the activity of multiple inositol polyphosphate phosphatase in vivo. In Molecular and cellular biology, 20, 6496-507. doi:. https://pubmed.ncbi.nlm.nih.gov/10938126/
7. Kilaparty, Surya P, Agarwal, Rakhee, Singh, Pooja, Kannan, Krishnaswamy, Ali, Nawab. 2016. Endoplasmic reticulum stress-induced apoptosis accompanies enhanced expression of multiple inositol polyphosphate phosphatase 1 (Minpp1): a possible role for Minpp1 in cellular stress response. In Cell stress & chaperones, 21, 593-608. doi:10.1007/s12192-016-0684-6. https://pubmed.ncbi.nlm.nih.gov/27038811/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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