C57BL/6JCya-Stk17bem1flox/Cya
Common Name
Stk17b-flox
Product ID
S-CKO-17308
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-98267-Stk17b-B6J-VA
Status
When using this mouse strain in a publication, please cite “Stk17b-flox Mouse (Catalog S-CKO-17308) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Stk17b-flox
Strain ID
CKOCMP-98267-Stk17b-B6J-VA
Gene Name
Product ID
S-CKO-17308
Gene Alias
Drak2, 3110009A03Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000027263
NCBI Transcript ID
NM_133810
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Overview of Gene Research
Stk17b, also known as DRAK2, is a serine/threonine protein kinase belonging to the death-associated protein kinase family. It is involved in various biological processes such as setting the threshold for T cell activation, regulating Purkinje cell dendritic development, and is associated with pathways like AKT/GSK-3β/Snail. It has been genetically linked to the development of diverse diseases, making it an important gene for understanding disease mechanisms [4,5,7].
In cancer research, Stk17b has been found to be highly expressed in ovarian cancer, hepatocellular carcinoma, and glioma tissues, promoting tumor cell proliferation, invasion, and migration. For example, in ovarian cancer, silencing Stk17b suppressed cell progression, while overexpression promoted it, with Stk17b increasing cell invasion and migration by promoting the EMT process [1]. In hepatocellular carcinoma, inhibition of Stk17b inhibited tumorigenesis and metastasis, and it was found to promote the EMT process via the AKT/GSK-3β/Snail signal pathway [3]. In glioma, methionine deprivation-induced inhibition of Stk17b suppressed cell proliferation and the EMT process [6]. In skin cutaneous melanoma, Stk17b had lower expression compared to normal tissues, and its low expression was correlated with poor overall survival and disease-specific survival, and it was also related to immune infiltration [2].
In conclusion, Stk17b plays a crucial role in multiple biological processes and disease conditions, especially in cancer progression. Its role in promoting tumorigenesis and metastasis in various cancers, as well as its association with immune-related processes in skin cutaneous melanoma, makes it a potential target for cancer diagnosis, prognosis, and immunotherapy. The findings from these functional studies help in understanding the complex mechanisms underlying these diseases and may lead to the development of novel therapeutic strategies.
References：
1. Jiang, Liping, Zhou, Jinhua, Zhao, Shaojie, Wang, Xuzhen, Chen, Youguo. . STK17B promotes the progression of ovarian cancer. In Annals of translational medicine, 9, 475. doi:10.21037/atm-21-601. https://pubmed.ncbi.nlm.nih.gov/33850872/
2. Shi, Xueying, Zhou, Qi, Huang, Bingqian, Fang, Shifeng, Lin, Jingrong. 2022. Prognostic and immune-related value of STK17B in skin cutaneous melanoma. In PloS one, 17, e0263311. doi:10.1371/journal.pone.0263311. https://pubmed.ncbi.nlm.nih.gov/35171924/
3. Lan, Yaliang, Han, Jihua, Wang, Yan, Subash, Sharma, Liu, Lianxin. 2018. STK17B promotes carcinogenesis and metastasis via AKT/GSK-3β/Snail signaling in hepatocellular carcinoma. In Cell death & disease, 9, 236. doi:10.1038/s41419-018-0262-1. https://pubmed.ncbi.nlm.nih.gov/29445189/
4. Scheuplein, Felix, Renner, Florian, Campbell, John E, Dorsch, Marion, Bischoff, James R. 2024. Evaluation of STK17B as a cancer immunotherapy target utilizing highly potent and selective small molecule inhibitors. In Frontiers in immunology, 15, 1411395. doi:10.3389/fimmu.2024.1411395. https://pubmed.ncbi.nlm.nih.gov/39502695/
5. Wu, Qin-Wei, Kapfhammer, Josef P. 2021. Serine/threonine kinase 17b (STK17B) signalling regulates Purkinje cell dendritic development and is altered in multiple spinocerebellar ataxias. In The European journal of neuroscience, 54, 6673-6684. doi:10.1111/ejn.15465. https://pubmed.ncbi.nlm.nih.gov/34536317/
6. Li, Jiafeng, Liu, Ruijie, Hu, Hong, Wang, Kaikai, Liu, Huailei. 2024. Methionine deprivation inhibits glioma proliferation and EMT via the TP53TG1/miR-96-5p/STK17B ceRNA pathway. In NPJ precision oncology, 8, 270. doi:10.1038/s41698-024-00763-y. https://pubmed.ncbi.nlm.nih.gov/39572759/
7. Liu, Chang, Zhang, Yichi, Zhang, Yuqing, Mao, Feifei, Chai, Zongtao. 2022. Mechanistic Insights into the Mechanism of Inhibitor Selectivity toward the Dark Kinase STK17B against Its High Homology STK17A. In Molecules (Basel, Switzerland), 27, . doi:10.3390/molecules27144655. https://pubmed.ncbi.nlm.nih.gov/35889528/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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