C57BL/6JCya-Nlrp5em1flox/Cya
Common Name
Nlrp5-flox
Product ID
S-CKO-18919
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-23968-Nlrp5-B6J-VA
Status
When using this mouse strain in a publication, please cite “Nlrp5-flox Mouse (Catalog S-CKO-18919) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Nlrp5-flox
Strain ID
CKOCMP-23968-Nlrp5-B6J-VA
Gene Name
Product ID
S-CKO-18919
Gene Alias
Op1, Mater, Nalp5, PAN11
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000015866
NCBI Transcript ID
NM_011860
Target Region
Exon 8
Size of Effective Region
~2.6 kb
Overview of Gene Research
Nlrp5, a maternal-effect gene, is crucial for normal pre-implantation and embryonic development [1,2,4,5,7,8]. It is part of the subcortical maternal complex (SCMC) and may be involved in stabilizing UHRF1 protein in the cytoplasm, which is relevant to maintaining CpG methylation of imprinting control regions during pre-implantation development [3].
In mouse models, Nlrp5 mutant oocytes have abnormal mitochondrial localization and increased activity, leading to reactive oxygen species accumulation, mitochondrial depletion, and early embryonic arrest at the two-cell stage. In sows, knockdown of NLRP5 arrests early embryogenesis [7,8]. In humans, mutations in NLRP5 are associated with female infertility, including oocyte maturation abnormality, total fertilization failure, and early embryonic arrest [2,5,9]. In sheep, certain mutations in NLRP5 are associated with litter size [6].
In conclusion, Nlrp5 plays an essential role in early embryogenesis across different species. Studies using gene-knockout or knockdown models in mice and sows, along with human genetic studies, have revealed its significance in processes like mitochondrial function regulation in oocytes and embryos, and its mutations are linked to infertility-related diseases.
References：
1. Huang, Xingchen, Sun, Qinqiang, Chen, Dongrong, Zhang, Ming, Fu, Qiang. 2022. Nlrp5 and Tle6 expression patterns in buffalo oocytes and pre-implantation embryos. In Reproduction in domestic animals = Zuchthygiene, 57, 481-488. doi:10.1111/rda.14084. https://pubmed.ncbi.nlm.nih.gov/35044003/
2. Tong, Xiaomei, Jin, Jiamin, Hu, Zhanhong, Zhang, Yin-Li, Zhang, Songying. 2022. Mutations in OOEP and NLRP5 identified in infertile patients with early embryonic arrest. In Human mutation, 43, 1909-1920. doi:10.1002/humu.24448. https://pubmed.ncbi.nlm.nih.gov/35946397/
3. Unoki, Motoko, Uemura, Shuhei, Fujimoto, Akihiro, Sasaki, Hiroyuki. . The maternal protein NLRP5 stabilizes UHRF1 in the cytoplasm: implication for the pathogenesis of multilocus imprinting disturbance. In Human molecular genetics, 33, 1575-1583. doi:10.1093/hmg/ddae096. https://pubmed.ncbi.nlm.nih.gov/38868925/
4. Sang, Qing, Zhou, Zhou, Mu, Jian, Wang, Lei. 2021. Genetic factors as potential molecular markers of human oocyte and embryo quality. In Journal of assisted reproduction and genetics, 38, 993-1002. doi:10.1007/s10815-021-02196-z. https://pubmed.ncbi.nlm.nih.gov/33895934/
5. Huang, Lingli, Wang, Yu, Lu, Fangting, Jin, Rentao, Tong, Xianhong. 2022. Novel mutations in NLRP5 and PATL2 cause female infertility characterized by primarily oocyte maturation abnormality and consequent early embryonic arrest. In Journal of assisted reproduction and genetics, 39, 711-718. doi:10.1007/s10815-022-02412-4. https://pubmed.ncbi.nlm.nih.gov/35091966/
6. Zhang, Zhuangbiao, Tang, Jishun, He, Xiaoyun, Di, Ran, Chu, Mingxing. 2020. Mutations in NLRP5 and NLRP9 are Associated with Litter Size in Small Tail Han Sheep. In Animals : an open access journal from MDPI, 10, . doi:10.3390/ani10040689. https://pubmed.ncbi.nlm.nih.gov/32326631/
7. Peng, Hui, Liu, Fang, Li, Wenhao, Zhang, Wenchang. 2015. Knockdown of NLRP5 arrests early embryogenesis in sows. In Animal reproduction science, 163, 151-6. doi:10.1016/j.anireprosci.2015.11.004. https://pubmed.ncbi.nlm.nih.gov/26585895/
8. Fernandes, Roxanne, Tsuda, Chiharu, Perumalsamy, Alagammal L, Nelson, Lawrence M, Jurisicova, Andrea. 2012. NLRP5 mediates mitochondrial function in mouse oocytes and embryos. In Biology of reproduction, 86, 138, 1-10. doi:10.1095/biolreprod.111.093583. https://pubmed.ncbi.nlm.nih.gov/22357545/
9. Li, Mingzhao, Jia, Miaomiao, Zhao, Xiaoli, Shi, Rong, Xue, Xia. 2020. A new NLRP5 mutation causes female infertility and total fertilization failure. In Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, 37, 283-284. doi:10.1080/09513590.2020.1832069. https://pubmed.ncbi.nlm.nih.gov/33073652/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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