C57BL/6JCya-Prss1em1/Cya
Common Name
Prss1-KO
Product ID
S-KO-15740
Backgroud
C57BL/6JCya
Strain ID
KOCMP-114228-Prss1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Prss1-KO Mouse (Catalog S-KO-15740) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Prss1-KO
Strain ID
KOCMP-114228-Prss1-B6J-VA
Gene Name
Product ID
S-KO-15740
Gene Alias
Try1, Try-1, Trygn16
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000031910
NCBI Transcript ID
NM_053243
Target Region
Exon 2~5
Size of Effective Region
~11.7 kb
Overview of Gene Research
PRSS1, encoding the cationic trypsinogen, is a crucial gene. Trypsinogen is an enzyme precursor that, when activated to trypsin, plays a key role in the digestive process by initiating the breakdown of proteins in the small intestine. Its abnormal activation or function can lead to pancreatitis, highlighting its importance in pancreatic health [1,2,3,4,5,6].
Mutations in PRSS1 cause human hereditary pancreatitis. Transgenic mice expressing mutant forms of human PRSS1, like PRSS1R122H, develop more severe pancreatitis upon induction by caerulein, lipopolysaccharide, ethanol, or a high-fat diet. Their pancreata show more DNA damage, apoptosis, collagen deposition, increased trypsin activity, and inflammatory cell infiltration, suggesting that mutant PRSS1 promotes inflammation and exacerbates pancreatitis [3].
In conclusion, PRSS1 is essential for normal pancreatic digestive function. Studies using transgenic mouse models expressing mutant PRSS1 have revealed its significant role in the development and severity of pancreatitis. Understanding PRSS1's function and its mutant-associated phenotypes in vivo helps in deciphering the pathogenesis of pancreatitis and may potentially lead to new therapeutic strategies for this disease [3].
References:
1. Girodon, Emmanuelle, Rebours, Vinciane, Chen, Jian Min, Ferec, Claude, Bienvenu, Thierry. 2020. Clinical interpretation of PRSS1 variants in patients with pancreatitis. In Clinics and research in hepatology and gastroenterology, 45, 101497. doi:10.1016/j.clinre.2020.07.004. https://pubmed.ncbi.nlm.nih.gov/33257277/
2. Zou, Wen-Bin, Tang, Xin-Ying, Zhou, Dai-Zhan, Chen, Jian-Min, Liao, Zhuan. 2018. SPINK1, PRSS1, CTRC, and CFTR Genotypes Influence Disease Onset and Clinical Outcomes in Chronic Pancreatitis. In Clinical and translational gastroenterology, 9, 204. doi:10.1038/s41424-018-0069-5. https://pubmed.ncbi.nlm.nih.gov/30420730/
3. Huang, Haojie, Swidnicka-Siergiejko, Agnieszka Katarzyna, Daniluk, Jaroslaw, Ji, Baoan, Logsdon, Craig D. 2019. Transgenic Expression of PRSS1R122H Sensitizes Mice to Pancreatitis. In Gastroenterology, 158, 1072-1082.e7. doi:10.1053/j.gastro.2019.08.016. https://pubmed.ncbi.nlm.nih.gov/31419436/
4. Masson, Emmanuelle, Chen, Jian-Min, Audrézet, Marie-Pierre, Cooper, David N, Férec, Claude. 2013. A conservative assessment of the major genetic causes of idiopathic chronic pancreatitis: data from a comprehensive analysis of PRSS1, SPINK1, CTRC and CFTR genes in 253 young French patients. In PloS one, 8, e73522. doi:10.1371/journal.pone.0073522. https://pubmed.ncbi.nlm.nih.gov/23951356/
5. Singh, Vikesh K, Yadav, Dhiraj, Garg, Pramod K. . Diagnosis and Management of Chronic Pancreatitis: A Review. In JAMA, 322, 2422-2434. doi:10.1001/jama.2019.19411. https://pubmed.ncbi.nlm.nih.gov/31860051/
6. Németh, Balázs Csaba, Sahin-Tóth, Miklós. 2014. Human cationic trypsinogen (PRSS1) variants and chronic pancreatitis. In American journal of physiology. Gastrointestinal and liver physiology, 306, G466-73. doi:10.1152/ajpgi.00419.2013. https://pubmed.ncbi.nlm.nih.gov/24458023/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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