C57BL/6JCya-Atf5em1/Cya
Common Name
Atf5-KO
Product ID
S-KO-17970
Backgroud
C57BL/6JCya
Strain ID
KOCMP-107503-Atf5-B6J-VA
Status
When using this mouse strain in a publication, please cite “Atf5-KO Mouse (Catalog S-KO-17970) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Atf5-KO
Strain ID
KOCMP-107503-Atf5-B6J-VA
Gene Name
Product ID
S-KO-17970
Gene Alias
AFTA, Atf7, Atfx, ODA-10
Background
C57BL/6JCya
Gene Full Name
activating transcription factor 5
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 7 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000107893
NCBI Transcript ID
NM_030693
Target Region
Exon 3
Size of Effective Region
~2.4 kb
Overview of Gene Research
Atf5, or Activating transcription factor 5, is a prosurvival transcription factor within the basic leucine zipper (bZip) family. It is involved in cellular differentiation and enables cells to adapt to stress. ATF5 is crucial in the mammalian mitochondrial unfolded protein response (UPRmt) through mitochondria-nuclear translocation, which helps maintain mitochondrial protein homeostasis during mitochondrial stress [1,4].
In various disease models, ATF5 shows diverse effects. In diabetic kidney disease (DKD), ATF5 initially has a protective effect, but in the long run, it promotes tubulointerstitial injury by regulating HSP60 and the UPRmt pathway. Inhibition of ATF5 in db/db mice improved serum creatinine, tubulointerstitial fibrosis, and apoptosis [1]. In cardiomyocyte studies, TMEM11 enhanced m7G methylation of Atf5 mRNA, increasing ATF5 expression, which suppressed cardiomyocyte proliferation [2]. In sepsis-induced liver injury, ASGR1 promoted monocyte-to-macrophage differentiation via the NF-κB/ATF5 pathway, and ASGR1-knockdown decreased ATF5 expression, alleviating liver injury [3]. In cutaneous T cell lymphoma, ATF5 overexpression promoted malignant T cell survival through the PI3K/AKT/mTOR pathway and conferred resistance to endoplasmic reticulum stress-induced apoptosis [5].
In conclusion, ATF5 is essential for maintaining mitochondrial function and cellular stress adaptation. Through gene-knockout or related models, its role in diseases such as DKD, cardiac repair, liver injury during sepsis, and cutaneous T cell lymphoma has been revealed. Understanding ATF5's functions in these disease conditions may provide potential therapeutic targets for treatment.
References：
1. Liu, Yifei, Zhang, Lei, Zhang, Shumin, Liu, Fuyou, Xiao, Li. 2023. ATF5 regulates tubulointerstitial injury in diabetic kidney disease via mitochondrial unfolded protein response. In Molecular medicine (Cambridge, Mass.), 29, 57. doi:10.1186/s10020-023-00651-4. https://pubmed.ncbi.nlm.nih.gov/37095454/
2. Chen, Xin-Zhe, Li, Xin-Min, Xu, Shi-Jun, Li, Pei-Feng, Wang, Kun. 2023. TMEM11 regulates cardiomyocyte proliferation and cardiac repair via METTL1-mediated m7G methylation of ATF5 mRNA. In Cell death and differentiation, 30, 1786-1798. doi:10.1038/s41418-023-01179-0. https://pubmed.ncbi.nlm.nih.gov/37286744/
3. Shi, Rui, Wang, Jiangang, Zhang, Zhen, Leng, Yiping, Chen, Alex F. 2023. ASGR1 promotes liver injury in sepsis by modulating monocyte-to-macrophage differentiation via NF-κB/ATF5 pathway. In Life sciences, 315, 121339. doi:10.1016/j.lfs.2022.121339. https://pubmed.ncbi.nlm.nih.gov/36621538/
4. Fiorese, Christopher J, Schulz, Anna M, Lin, Yi-Fan, Pellegrino, Mark W, Haynes, Cole M. 2016. The Transcription Factor ATF5 Mediates a Mammalian Mitochondrial UPR. In Current biology : CB, 26, 2037-2043. doi:10.1016/j.cub.2016.06.002. https://pubmed.ncbi.nlm.nih.gov/27426517/
5. Cao, Mengzhou, Lai, Pan, Liu, Xiangjun, Tu, Ping, Wang, Yang. 2023. ATF5 promotes malignant T cell survival through the PI3K/AKT/mTOR pathway in cutaneous T cell lymphoma. In Frontiers in immunology, 14, 1282996. doi:10.3389/fimmu.2023.1282996. https://pubmed.ncbi.nlm.nih.gov/38223508/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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