C57BL/6JCya-Sgf29em1/Cya
Common Name
Sgf29-KO
Product ID
S-KO-18881
Backgroud
C57BL/6JCya
Strain ID
KOCMP-75565-Sgf29-B6J-VB
Status
When using this mouse strain in a publication, please cite “Sgf29-KO Mouse (Catalog S-KO-18881) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Sgf29-KO
Strain ID
KOCMP-75565-Sgf29-B6J-VB
Gene Name
Product ID
S-KO-18881
Gene Alias
Ccdc101, 1700023O11Rik, 9530025I05Rik
Background
C57BL/6JCya
Gene Full Name
SAGA complex associated factor 29
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 7 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000032956
NCBI Transcript ID
NM_029339
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Overview of Gene Research
Sgf29, a component of the SAGA transcriptional coactivator complex, is involved in crucial biological processes. It binds to histone H3K4me2/3, facilitating SAGA complex recruitment and histone H3 acetylation, which is important for gene regulation [4]. It also participates in histone post-translational modification as part of the Ada2/Ada3/Gcn5/Sgf29 histone acetyltransferase module [2].
In cellular senescence of human mesenchymal progenitor cells and fibroblasts, Sgf29 forms liquid-like nuclear condensates. The Arg 207 in its intrinsically disordered region is key for phase separation. These condensates, along with H3K4me3 binding, are essential for its chromatin location, recruitment of transcriptional factors, and activation of senescence-associated genes like CDKN1A [1]. In acute myeloid leukemia (AML), Sgf29 deletion impaired leukemogenesis in multiple subtype models, revealing its role in the transcription of AML oncogenes [3]. A CRISPR-based study identified Sgf29 as crucial for H3K9 acetylation, ribosomal gene expression, and leukemogenesis, and developed a drug discovery strategy targeting its Tudor domain [5].
In summary, Sgf29 is vital for gene regulation through histone modification and phase-separation-related transcriptional regulation. Its study in AML and cellular aging models has provided insights into disease mechanisms. Understanding Sgf29's functions may offer potential therapeutic targets for leukemia and aging-related research.
References：
1. Yan, Kaowen, Ji, Qianzhao, Zhao, Dongxin, Zhang, Weiqi, Liu, Guang-Hui. 2023. SGF29 nuclear condensates reinforce cellular aging. In Cell discovery, 9, 110. doi:10.1038/s41421-023-00602-7. https://pubmed.ncbi.nlm.nih.gov/37935676/
2. Espinola-Lopez, Jose M, Tan, Song. 2020. The Ada2/Ada3/Gcn5/Sgf29 histone acetyltransferase module. In Biochimica et biophysica acta. Gene regulatory mechanisms, 1864, 194629. doi:10.1016/j.bbagrm.2020.194629. https://pubmed.ncbi.nlm.nih.gov/32890768/
3. Barbosa, Karina, Deshpande, Anagha, Perales, Marlenne, Adams, Peter D, Deshpande, Aniruddha J. . Transcriptional control of leukemogenesis by the chromatin reader SGF29. In Blood, 143, 697-712. doi:10.1182/blood.2023021234. https://pubmed.ncbi.nlm.nih.gov/38048593/
4. Bian, Chuanbing, Xu, Chao, Ruan, Jianbin, Zang, Jianye, Min, Jinrong. 2011. Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation. In The EMBO journal, 30, 2829-42. doi:10.1038/emboj.2011.193. https://pubmed.ncbi.nlm.nih.gov/21685874/
5. Chan, Anthony K N, Han, Li, Delaney, Christopher D, Su, Rui, Chen, Chun-Wei. 2024. Therapeutic targeting Tudor domains in leukemia via CRISPR-Scan Assisted Drug Discovery. In Science advances, 10, eadk3127. doi:10.1126/sciadv.adk3127. https://pubmed.ncbi.nlm.nih.gov/38394203/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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