C57BL/6JCya-Ghrem1/Cya
Common Name
Ghr-KO
Product ID
S-KO-19378
Backgroud
C57BL/6JCya
Strain ID
KOCMP-14600-Ghr-B6J-VB
Status
When using this mouse strain in a publication, please cite “Ghr-KO Mouse (Catalog S-KO-19378) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ghr-KO
Strain ID
KOCMP-14600-Ghr-B6J-VB
Gene Name
Product ID
S-KO-19378
Gene Alias
GHBP, GHR/BP
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 15 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000069451
NCBI Transcript ID
NM_010284
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Overview of Gene Research
Ghr, the growth hormone receptor, is a membrane-bound receptor belonging to the class I cytokine receptor superfamily. Growth hormone (GH) binding to Ghr induces cell differentiation, maturation, anabolism, and proliferation. The GH-GHR-IGF1 axis activates the JAK2/STAT5 pathway to stimulate IGF1 synthesis, playing significant roles in somatic growth, such as cell proliferation, differentiation, division, and survival [2,3]. Genetic models, like gene knockout mice, are valuable for studying Ghr's functions.
In GHR-/-mice, there are decreased body weights but obesity. Despite obesity, these mice seem protected from age-related white adipose tissue inflammation and immune cell infiltration, with modest and depot-specific changes to immune cell populations in white adipose tissue (WAT) depots [4]. In another study, knockdown of GHR in HCC cells enhanced their sensitivity to sorafenib, potentially via inhibiting the PI3K/AKT/ERK1/2 signaling pathway [1]. Also, GHR deficiency in gastric cancer cells inhibited cell growth and induced apoptosis, arresting cells in the G1 phase, and was associated with inactivation of the PI3K/AKT signaling pathway [2].
In conclusion, Ghr is essential for mediating the effects of growth hormone, influencing multiple biological processes including cell growth, metabolism, and immune cell regulation in adipose tissue. Gene knockout models of Ghr have provided insights into its role in diseases such as cancer and obesity-related inflammation, contributing to our understanding of disease mechanisms and potentially guiding future therapeutic strategies.
References：
1. Gao, Shuang, Ni, Qianwen, Wu, Xiuli, Cao, Tieliu. 2020. GHR knockdown enhances the sensitivity of HCC cells to sorafenib. In Aging, 12, 18127-18136. doi:10.18632/aging.103625. https://pubmed.ncbi.nlm.nih.gov/32970612/
2. Yan, Hong-Zhu, Wang, Hua-Feng, Yin, Yueling, He, Ying, He, Bo-Sheng. 2021. GHR is involved in gastric cell growth and apoptosis via PI3K/AKT signalling. In Journal of cellular and molecular medicine, 25, 2450-2458. doi:10.1111/jcmm.16160. https://pubmed.ncbi.nlm.nih.gov/33492754/
3. Hu, Bowen, Li, Hongmei, Zhang, Xiquan. 2021. A Balanced Act: The Effects of GH-GHR-IGF1 Axis on Mitochondrial Function. In Frontiers in cell and developmental biology, 9, 630248. doi:10.3389/fcell.2021.630248. https://pubmed.ncbi.nlm.nih.gov/33816476/
4. Young, Jonathan A, Henry, Brooke E, Benencia, Fabian, Kopchick, John J, Berryman, Darlene E. 2020. GHR-/- Mice are protected from obesity-related white adipose tissue inflammation. In Journal of neuroendocrinology, 32, e12854. doi:10.1111/jne.12854. https://pubmed.ncbi.nlm.nih.gov/32350959/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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