Engineered nuclease-mediated genome editing, especially CRISPR, has emerged as a promising technology which can serve as an alternative to the conventional, embryonic stem (ES) cell homologous recombination-based generation of animal models.

CRISPR, which stands for “Clustered regularly interspaced short palindromic repeats,” works alongside Cas proteins to form the CRISPR-Cas complex – a biological technology originally derived from a prokaryotic adaptive immunity system. To show how this process works, our video introduces the individual components of a CRISPR-Cas complex using the SHANK3 gene as an example.

 

Video: The Basics of Crispr-Cas9 Knockout Mice

Some people may think that gene knockout (KO) models are mostly developed using traditional embryonic stem (ES) cell technology, however, CRISPR has become widely used to generate these KO animal models due to a faster turnaround time, lower cost, and higher targeting efficiency has led to its widespread use in the industry. Using the SHANK3 gene as an example, this video shows you how scientists use CRISPR-Cas9 to generate knockouts in mice as well as other animals.

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CRISPR-Related Resources:

CRISPR-Cas9: How the Patent Dispute has Transformed Science Innovation

AAV-CRISPR/Cas9-Mediated Gene Editing - An Efficient Tool for Atherosclerosis Research

 

About Cyagen:

In under 15 years since its founding, Cyagen has become a leading provider of custom mouse and rat models – delivering over 78,400 models to researchers worldwide and receiving over 4,800 peer-reviewed citations. Cyagen prides itself on its premium customer service: including price-matching, client access to complimentary technical consultations, full confidentiality, and a 100% money-back service guarantee.

 

Why Choose Cyagen?

1. Complete research solutions – from custom rodent model generation to therapeutic viral packaging and injection.

2. Both in vivo (animal) and in vitro (cell) experimental models available

3. Expert scientific team provides in-depth technical support in formulating research model strategies.

 

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If you are engaged with or interested in gene therapy research, Cyagen’s experts can provide you with complimentary custom model generation strategies, helping develop your gene therapy research and publish high-impact articles.

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