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Citations
IMMUNITY 47:107 (2017) IF=22.845
Clec4f-iCre Mice
Catalog Number：C001353
Genetic Background：C57BL/6J
Reproduction：Homozygote x Homozygote
Strain Description
CLEC4F (C-Type Lectin Domain Family 4 Member F) is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Studies have shown that CLEC4F is a highly glycosylated membrane protein co-expressed with F4/80 on Kupffer cells (KC) and mCLEC4F was detected in the fetal liver at embryonic day 11.5 (E11.5). Kupffer cells are resident macrophages in the liver that participate in iron metabolism and the clearance of gut-derived microbial products.
This strain was constructed using gene editing technology. The “IRES-iCre” cassette was inserted 60~100 bp down stream of the TAG stop codon. When crossed with mice containing loxP sites, the offspring can produce sequence recombination between loxP sites mediated by Cre recombinase in KC cells. The homozygous Clec4f-iCre mice are viable and fertile.
The “IRES-iCre” cassette was inserted 60~100 bp down stream of the TAG stop codon.
a. Method
The Clec4f-iCre mice were bred with Rosa26-LSL-tdTomato mice to generate double heterozygous offspring. Cre-mediated recombination will result in the expression of tdTomato protein in the Cre-positive cells of the offspring. The liver tissue was collected from the offspring mice and immunofluorescence staining was used to observe the expression of tdTomato protein to determine the activity of Cre recombinase.
b. Genotype
Cre+: Clec4f-iCre[KI/+]; Rosa26-LSL-tdTomato[CKI/+]
Cre-: Rosa26-LSL-tdTomato[CKI/+]
c. Result
Figure 1. Immunofluorescence of the liver tissues. The results showed that the red fluorescence from tdTomato was completely overlapped with the green F4/80 labeling, indicating that Cre recombination occurred in these cells. In contrast, no red fluorescence signal was detected in the control group (Cre- mice).
d.Summary
In summary, this model has significant Cre recombination in the Kupffer cells, co-localized with the macrophage marker F4/80, and has good expression specificity.
The Cre recombinase gene is located on chromosome 6 in Clec4f-iCre mice. Please avoid breeding with gene-edited mice targeting the same chromosome as the Cre mice.
