The DBA/1-hTNF mice is a mouse Tnf gene humanized model. The mouse Tnf gene in the DBA/1 strain is replaced with the human TNF gene, including the 5’UTR and 3’UTR. This model is useful for diseases researches such as rheumatoid arthritis (RA). Since immunization of DBA/1 mice with type II collagen leads to severe polyarthritis mediated by the autoimmune response, DBA/1 mice are widely used for RA model construction. Therefore, DBA/1-hTNF mice constructed on a DBA/1 strain background can be used to research immune-related diseases such as RA.