The B6-hIL7R mouse is a humanized model constructed using gene editing technology, where the mouse IL7R endogenous extracellular domain (aa.21~239) was replaced with the human IL7R extracellular domain (aa.21~239). The murine IL7R signal peptide and cytoplasmic region (aa.1~20, aa.240~459) was preserved. Homozygous B6-hIL7R mice are viable and fertile. This model can be used for studying the pathological mechanisms and therapeutic approaches of autoimmune diseases, immunodeficiency, and cancer, and for the development of IL7R-targeted drugs.