The B6-hITGB7 mouse is a humanized model constructed using gene editing technology, where the mouse Itgb7 endogenous extracellular domain (aa.20~724) was replaced with the human ITGB7 extracellular domain (aa.20~723). The murine signal peptide (aa.1~19) was preserved. This model can be used for studying the pathological mechanisms and therapeutic approaches of inflammatory and autoimmune diseases, and for the development of ITGB7-targeted drugs.