B6-hURAT1 mice are humanized models in which the sequences from ~5 kb upstream of exon 1 to exon 10 of the mouse Slc22a12 gene were replaced with the sequences from ~5 kb upstream of exon 1 to exon 10 of the human SLC22A12 gene. This model can be used to study the pathological mechanisms and therapeutic methods of renal hypouricemia, gout, and other uric acid metabolism-related diseases, as well as the screening and development of URAT1-targeted drugs, and preclinical efficacy and safety evaluations.