huC5AR1 Mouse
Product Name
huC5AR1 Mouse
Product ID
C001714
Strain Name
C57BL/6NCya-C5ar1tm1(hC5AR1)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “huC5AR1 Mouse (Catalog C001714) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
C5AR1
Gene Alias
C5A, C5AR, C5R1, CD88
NCBI ID
Chromosome
Chr 19 (Human)
MGI ID
Datasheet
Strain Description
The gene C5AR1 encodes the complement component 5a receptor 1 (C5aR1, CD88), a principal member of the G protein-coupled receptor (GPCR) family. C5aR1 functions as the high-affinity receptor for the potent complement anaphylatoxin C5a, transducing its inflammatory and chemotactic signals [1]. The receptor exhibits broad expression, particularly enriched on cells of the innate immune system, including neutrophils, monocytes, macrophages, and dendritic cells, with detectable expression also noted on endothelial, epithelial, and smooth muscle cells [2]. Upon engagement with C5a, C5aR1 couples to G proteins and recruits β-arrestin, activating intracellular signaling cascades that orchestrate diverse cellular responses such as directed migration, degranulation, oxidative burst, and the synthesis of pro-inflammatory mediators [2-3]. Dysregulated C5aR1 signaling is a critical factor in the pathogenesis of numerous inflammatory and autoimmune disorders, including sepsis, rheumatoid arthritis, inflammatory bowel disease, and psoriasis, and is implicated in neuroinflammatory conditions such as Alzheimer's disease, as well as contributing to the tumor microenvironment [3-4]. Given its pivotal role in mediating C5a-driven inflammation, C5aR1 has emerged as a compelling therapeutic target, with pharmacological modulators currently undergoing investigation for various clinical applications.
The huC5AR1 mouse is a humanized model constructed by replacing the partial intron 1 to TAG stop codon of the mouse C5ar1 gene in situ with the corresponding sequence from the human C5AR1 gene. huC5AR1 mice can be used for research on the pathogenesis of various inflammatory diseases, such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease, neuroinflammation related to Alzheimer's disease, and some tumors, as well as for C5AR1-targeted drug development.
Reference
Feng Y, Zhao C, Deng Y, Wang H, Ma L, Liu S, Tian X, Wang B, Bin Y, Chen P, Yan W, Fu P, Shao Z. Mechanism of activation and biased signaling in complement receptor C5aR1. Cell Res. 2023 Apr;33(4):312-324.
Ruocco A, Sirico A, Novelli R, Iannelli S, Van Breda SV, Kyburz D, Hasler P, Aramini A, Amendola PG. The role of C5a-C5aR1 axis in bone pathophysiology: A mini-review. Front Cell Dev Biol. 2022 Aug 8;10:957800.
Carvalho K, Schartz ND, Balderrama-Gutierrez G, Liang HY, Chu SH, Selvan P, Gomez-Arboledas A, Petrisko TJ, Fonseca MI, Mortazavi A, Tenner AJ. Modulation of C5a-C5aR1 signaling alters the dynamics of AD progression. J Neuroinflammation. 2022 Jul 11;19(1):178.
Ding P, Xu Y, Li L, Lv X, Li L, Chen J, Zhou D, Wang X, Wang Q, Zhang W, Liao T, Ji QH, Lei QY, Hu W. Intracellular complement C5a/C5aR1 stabilizes β-catenin to promote colorectal tumorigenesis. Cell Rep. 2022 May 31;39(9):110851.
Strain Strategy
The partial intron 1 to stop codon of mouse C5ar1 was replaced with the partial intron 1 to stop codon of human C5AR1.
Figure 1. Gene editing strategy of huC5AR1 Mice.
Application Area
C5AR1-targeted drug screening, development, and evaluation;
Research on the pathological mechanisms and therapeutic approaches of various inflammatory diseases and autoimmune diseases, such as psoriasis, rheumatoid arthritis, and inflammatory bowel disease;
Research on the neuroinflammation related to Alzheimer's disease and some tumors.
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