B6-hFAP Mouse
Product Name
B6-hFAP Mouse
Product ID
C001783
Strain Name
C57BL/6NCya-Faptm1(hFAP)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “B6-hFAP Mouse (Catalog C001783) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
FAP
Gene Alias
FAPA, SIMP, DPPIV, FAPalpha
NCBI ID
Chromosome
Chr 2 (Human)
MGI ID
Datasheet
Strain Description
The FAP gene (Fibroblast Activation Protein alpha) encodes a homodimeric integral membrane serine protease that plays a crucial role in extracellular matrix degradation and various cellular processes, including tissue remodeling, wound healing, fibrosis, and tumor growth [1]. While its expression is typically very low in normal adult tissues, FAP is selectively and highly expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas [2]. The protein functions as both a dipeptidyl peptidase and an endopeptidase, cleaving specific sequences in various bioactive peptides and structural proteins, such as neuropeptide Y and denatured collagen [3]. This specific expression pattern makes FAP a promising target for imaging and therapy in oncology, as it labels cancer-associated fibroblasts (CAFs) in over 90% of human carcinomas [4].
The B6-hFAP mouse is a humanized model constructed by replacing the sequence from ATG start codon to partial intron 2 of the mouse Fap gene with the Kozak Human FAP CDS-3'UTR of Mouse Fap-WPRE-BGH pA cassette. B6-hFAP mice can be used for research into the pathogenesis of various malignant tumors, as well as for the screening, development, and safety evaluation of FAP-targeted drugs.
Reference
Fitzgerald AA, Weiner LM. The role of fibroblast activation protein in health and malignancy. Cancer Metastasis Rev. 2020 Sep;39(3):783-803.
Hamson EJ, Keane FM, Tholen S, Schilling O, Gorrell MD. Understanding fibroblast activation protein (FAP): substrates, activities, expression and targeting for cancer therapy. Proteomics Clin Appl. 2014 Jun;8(5-6):454-63.
Shahvali S, Rahiman N, Jaafari MR, Arabi L. Targeting fibroblast activation protein (FAP): advances in CAR-T cell, antibody, and vaccine in cancer immunotherapy. Drug Deliv Transl Res. 2023 Jul;13(7):2041-2056.
Dendl K, Koerber SA, Kratochwil C, Cardinale J, Finck R, Dabir M, Novruzov E, Watabe T, Kramer V, Choyke PL, Haberkorn U, Giesel FL. FAP and FAPI-PET/CT in Malignant and Non-Malignant Diseases: A Perfect Symbiosis? Cancers (Basel). 2021 Sep 30;13(19):4946.
Strain Strategy
The sequence from ATG start codon to partial intron 2 of mouse Fap will be replaced with the Kozak Human FAP CDS-3'UTR of Mouse Fap-WPRE-BGH pA cassette.
Figure 1. Gene editing strategy of B6-hFAP Mice.
Application Area
FAP-targeted drug screening, development, and evaluation;
Research on the pathological mechanisms and therapeutic approaches of malignant tumors.
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