huAPOE3 Mouse
Product Name
huAPOE3 Mouse
Product ID
C002021
Strain Name
C57BL/6JCya-Apoeem2(hAPOEε3)/Cya
Backgroud
C57BL/6JCya
Status
When using this mouse strain in a publication, please cite “huAPOE3 Mouse (Catalog C002021) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
APOE
Gene Alias
AD2, LPG, APO-E, ApoE4, LDLCQ5
NCBI ID
Chromosome
Chr 19 (Human)
MGI ID
Datasheet
Strain Description
The APOE3 (Apolipoprotein E epsilon 3) gene represents the most prevalent isoform within the human population and is typically categorized as the functionally "neutral" or wild-type allele. Predominantly expressed in the liver and central nervous system, it is produced by hepatocytes and astrocytes to encode the 299-amino acid Apolipoprotein E glycoprotein [1]. This protein serves as a vital ligand for LDL receptors, facilitating the systemic transport and redistribution of cholesterol and triglycerides necessary for membrane stability and neural synaptic repair. While APOE3 generally supports healthy lipid homeostasis, the APOE4 isoform is associated with increased risk of cardiovascular pathologies like atherosclerosis, whereas APOE2 homozygosity (or rare APOE variants) can lead to familial dysbetalipoproteinemia in specific genetic contexts [2]. Furthermore, it serves as the baseline for assessing neurodegenerative risk, sitting between the neuroprotective effects of the APOE2 variant and the significantly increased Alzheimer's disease risk associated with APOE4 [3].
The huAPOE3 mouse is a humanized model constructed by using gene-editing technology to replace exons 2-4 and part of the flanking sequences of the mouse Apoe gene with the human APOE gene sequences, including exons 2, 3, 4, and some downstream sequence of 3’UTR. This model can be used for research on cardiovascular diseases, such as atherosclerosis, and neurodegenerative diseases, such as Alzheimer's disease (AD), as well as for the development of APOE3-targeted drugs.
Reference
Budny V, Ruminot I, Wybitul M, Treyer V, Barros LF, Tackenberg C. Fueling the brain - the role of apolipoprotein E in brain energy metabolism and its implications for Alzheimer's disease. Transl Psychiatry. 2025 Aug 25;15(1):316.
Liu C, Liu J, Wang YY, Xu SF, Yu LM. APOE Lipoprotein Particles: Pathophysiology, Therapy, and the Crosstalk in Alzheimer's Disease and Cardiovascular Disease. Mol Neurobiol. 2025 Dec 23;63(1):325.
Belaidi AA, Bush AI, Ayton S. Apolipoprotein E in Alzheimer's disease: molecular insights and therapeutic opportunities. Mol Neurodegener. 2025 Apr 24;20(1):47.
Strain Strategy
Exons 2-4 plus partial flanking sequences of the mouse Apoe gene were replaced with the human APOE gene sequence, including exons 2, 3, and 4 and some downstream sequence of 3’UTR.
Figure 1. Diagram of the gene editing strategy for the generation of huAPOE3 mice.
Application Area
Screening, development, and preclinical evaluation of APOE3-targeted drugs;
Research on the pathological mechanisms and treatment methods of cardiovascular diseases, such as atherosclerosis;
Research on the pathological mechanisms and treatment methods of neurodegenerative diseases, such as Alzheimer's disease (AD).
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
