Sox9-IRES-CreERT2 Mouse
Product Name
Sox9-IRES-CreERT2 Mouse
Product ID
C002059
Strain Name
C57BL/6JCya-Sox9em1(IRES-CreERT2)/Cya
Backgroud
C57BL/6JCya
Examples of Expressing Tissues/Cells
Cartilage tissue, testicular Sertoli cells, genital ridge, and Sox9-positive cells
Status
When using this mouse strain in a publication, please cite “Sox9-IRES-CreERT2 Mouse (Catalog C002059) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Sox9
Gene Alias
mSox9, mKIAA4243, 2010306G03Rik
NCBI ID
Chromosome
Chr 11 (Mouse)
MGI ID
Datasheet
Strain Description
SRY-Box Transcription Factor 9 (SOX9) is a member of the high-mobility group (HMG) superfamily transcription factors encoded by the Sox9 gene. SOX9 is highly expressed in chondrocytes and is also widely expressed in Sertoli cells, genital ridges, kidneys, hearts, and multiple other tissues [1-2]. SOX9 is an important master regulatory transcription factor during chondrogenesis and participates in cartilage matrix synthesis and skeletal development through regulating the transcription of cartilage-associated genes, including Col2a1 and Agc1 [2-3]. In addition, SOX9 also plays important roles in gonadal development and maintenance of the germ cell microenvironment [1,4]. Studies have shown that aberrations in Sox9 are closely associated with skeletal and gonadal developmental disorders, including campomelic dysplasia and sex reversal syndrome. Therefore, SOX9 has been widely used in studies of skeletal development, reproductive development, and regenerative medicine [3-4].
Sox9-IRES-CreERT2 mice were generated by knocking a tamoxifen-inducible CreERT2 cassette into the endogenous Sox9 locus. Without tamoxifen, CreERT2 is retained in the cytoplasm; upon induction, it translocates to the nucleus to mediate recombination. When crossed with mice containing loxP sites, Cre-mediated recombination is expected to occur in Sox9-positive cells of the offspring after tamoxifen induction.
Reference
Koopman P, Gubbay J, Vivian N, et al. Male development of chromosomally female mice transgenic for Sry. Nature. 1991;351(6322):117-121.
Akiyama H, Chaboissier MC, Martin JF, et al. The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev. 2002;16(21):2813-2828.
Bi W, Deng JM, Zhang Z, et al. Sox9 is required for cartilage formation. Nat Genet. 1999;22(1):85-89.
Foster JW, Dominguez-Steglich MA, Guioli S, et al. Campomelic dysplasia and autosomal sex reversal caused by mutations in an SRY-related gene. Nature. 1994;372(6506):525-530.
Strain Strategy
The IRES-CreERT2-SV40 late pA cassette was inserted downstream of the stop codon.
Figure 1. Gene editing strategy of Sox9-IRES-CreERT2 mice.
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