C57BL/6JCya-Cyp2j11em1flox/Cya
Common Name
Cyp2j11-flox
Product ID
S-CKO-00062
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-100066-Cyp2j11-B6J-VA
Status
When using this mouse strain in a publication, please cite “Cyp2j11-flox Mouse (Catalog S-CKO-00062) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Cyp2j11-flox
Strain ID
CKOCMP-100066-Cyp2j11-B6J-VA
Gene Name
Product ID
S-CKO-00062
Gene Alias
Cyp2j11-ps
Background
C57BL/6JCya
Gene Full Name
cytochrome P450, family 2, subfamily j, polypeptide 11
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 4 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000015368
NCBI Transcript ID
NM_001004141
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Overview of Gene Research
Cyp2j11 is a gene belonging to the cytochrome P450 CYP2J subfamily. Members of this subfamily are involved in the metabolism of fatty acids, generating bioactive compounds. They participate in processes such as the metabolism of arachidonic acid (AA), linoleic acid (LA), and other lipids and xenobiotics [1,4].
Quantitative polymerase chain reaction (qPCR) analysis showed that Cyp2j11 was highly expressed in both male and female mouse kidneys [1]. Tissue distribution studies also found that Cyp2j11 transcripts were most abundant in the kidney and heart, with protein detected primarily in the kidney (proximal convoluted tubules), liver, and heart (cardiomyocytes) [4]. In addition, sex differences in Cyp2j11 mRNA levels were observed in both wild-type and GHR deficient mice, and the constant GH infusion induced heart Cyp2j11 in male C57/B6 mice [2]. HL-1 cells expressed Cyp2j11 to the same extent as mouse hearts, and these cells can metabolize AA to various products, suggesting a role in cardiovascular-related AA metabolism [3]. Also, in a study on selenium against liver injury induced by beta-cypermethrin in mice, Cyp2j11 was significantly enriched in functional categories related to retinol metabolism, linoleic acid metabolism, and Jak-STAT signaling pathway [5].
In conclusion, Cyp2j11 is significantly expressed in multiple tissues like the kidney, heart, and liver, and is involved in fatty acid metabolism, especially AA and LA metabolism. Its expression shows sex-related differences and is associated with some liver injury-related pathways. The study of Cyp2j11 in mouse models helps to understand its role in these biological processes and related disease conditions.
References：
1. Graves, Joan P, Gruzdev, Artiom, Bradbury, J Alyce, Edin, Matthew L, Zeldin, Darryl C. 2015. Quantitative Polymerase Chain Reaction Analysis of the Mouse Cyp2j Subfamily: Tissue Distribution and Regulation. In Drug metabolism and disposition: the biological fate of chemicals, 43, 1169-80. doi:10.1124/dmd.115.064139. https://pubmed.ncbi.nlm.nih.gov/25994032/
2. Zhang, Furong, Yu, Xuming, He, Chunyan, Wan, Yu, Yue, Jiang. 2015. Effects of sexually dimorphic growth hormone secretory patterns on arachidonic acid metabolizing enzymes in rodent heart. In Toxicology and applied pharmacology, 289, 495-506. doi:10.1016/j.taap.2015.10.011. https://pubmed.ncbi.nlm.nih.gov/26493931/
3. Elshenawy, Osama H, Anwar-Mohamed, Anwar, Abdelhamid, Ghada, El-Kadi, Ayman O S. 2012. Murine atrial HL-1 cell line is a reliable model to study drug metabolizing enzymes in the heart. In Vascular pharmacology, 58, 326-33. doi:10.1016/j.vph.2012.12.002. https://pubmed.ncbi.nlm.nih.gov/23268359/
4. Graves, Joan P, Edin, Matthew L, Bradbury, J Alyce, Tomer, Kenneth B, Zeldin, Darryl C. 2013. Characterization of four new mouse cytochrome P450 enzymes of the CYP2J subfamily. In Drug metabolism and disposition: the biological fate of chemicals, 41, 763-73. doi:10.1124/dmd.112.049429. https://pubmed.ncbi.nlm.nih.gov/23315644/
5. He, Kan, Tang, Qingyang, Gong, Mengting, Liu, Dahai, Huang, Bei. 2019. A transcriptomic study of selenium against liver injury induced by beta-cypermethrin in mice by RNA-seq. In Functional & integrative genomics, 20, 343-353. doi:10.1007/s10142-019-00719-7. https://pubmed.ncbi.nlm.nih.gov/31659573/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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