C57BL/6JCya-Rrp8em1flox/Cya
Common Name:
Rrp8-flox
Product ID:
S-CKO-00240
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Rrp8-flox
Strain ID
CKOCMP-101867-Rrp8-B6J-VA
Gene Name
Product ID
S-CKO-00240
Gene Alias
1500003O22Rik; 2900001K19Rik; NML
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rrp8em1flox/Cya mice (Catalog S-CKO-00240) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098148
NCBI RefSeq
NM_133951
Target Region
Exon 2~7
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Rrp8, ribosomal RNA processing 8, is a nucleolar Rossman fold-like methyltransferase involved in RNA modification, specifically responsible for N1-methyladenosine (m1A) base modification at position 645 of 25S rRNA in yeast, corresponding to modifications in other species like Caenorhabditis elegans [3,4]. It has been associated with ribosomal biogenesis, including A2 cleavage and 40S biogenesis in yeast [4].
In hepatocellular carcinoma (HCC), Rrp8 is markedly overexpressed compared to adjacent normal tissues. Knockdown and knockout of Rrp8 in HCC cells attenuated cell proliferation and migration. Knockout also decreased the phosphorylation of MEK1/2 and β-catenin-(Y654) signalling pathway components, downregulated downstream effectors like Cyclin D1 and N-cadherin, and upregulated E-cadherin. Rrp8 is strongly associated with immune infiltration and various immune markers in HCC, and elevated expression indicates an unfavourable prognosis [1]. In podocytes, loss of Profilin1 led to downregulation of Rrp8, resulting in mitotic catastrophe. Overexpression of Rrp8 in Pfn1-KO podocytes partially rescued the phenotype in vitro [2].
In conclusion, Rrp8 plays a crucial role in RNA modification and ribosomal biogenesis. Its dysregulation is associated with diseases such as HCC and glomerular diseases. Studies using knockout models in these contexts have revealed its functions in cell proliferation, migration, and immune infiltration, providing potential therapeutic targets for these diseases.
References:
1. You, Kai, Du, Xingxing, Zhao, Yunzheng, Lu, Zhaoyang, Fan, Huitao. 2024. RRP8, associated with immune infiltration, is a prospective therapeutic target in hepatocellular carcinoma. In Journal of cancer research and clinical oncology, 150, 245. doi:10.1007/s00432-024-05756-9. https://pubmed.ncbi.nlm.nih.gov/38722372/
2. Tian, Xuefei, Pedigo, Christopher E, Li, Ke, Fu, Rongguo, Ishibe, Shuta. 2023. Profilin1 is required for prevention of mitotic catastrophe in murine and human glomerular diseases. In The Journal of clinical investigation, 133, . doi:10.1172/JCI171237. https://pubmed.ncbi.nlm.nih.gov/37847555/
3. Yokoyama, Wataru, Hirota, Keiko, Wan, Huahua, Kako, Koichiro, Fukamizu, Akiyoshi. . rRNA adenine methylation requires T07A9.8 gene as rram-1 in Caenorhabditis elegans. In Journal of biochemistry, 163, 465-474. doi:10.1093/jb/mvy018. https://pubmed.ncbi.nlm.nih.gov/29385568/
4. Peifer, Christian, Sharma, Sunny, Watzinger, Peter, Kötter, Peter, Entian, Karl-Dieter. 2012. Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA. In Nucleic acids research, 41, 1151-63. doi:10.1093/nar/gks1102. https://pubmed.ncbi.nlm.nih.gov/23180764/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen