C57BL/6JCya-Plin1em1flox/Cya
Common Name:
Plin1-flox
Product ID:
S-CKO-00350
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Plin1-flox
Strain ID
CKOCMP-103968-Plin1-B6J-VA
Gene Name
Product ID
S-CKO-00350
Gene Alias
6030432J05Rik; Peri; Plin
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Plin1em1flox/Cya mice (Catalog S-CKO-00350) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000205915
NCBI RefSeq
NM_001113471
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Plin1, encoding perilipin-1, is a member of the PAT protein family related to lipid droplet surface proteins. It coats lipid droplets in adipocytes, playing a vital role in droplet formation, triglyceride storage, and lipolysis. Phosphorylation of PLIN1 is crucial in adipose tissue lipolysis and fat storage in adipocytes. It is also involved in pathways like AMPK, Wnt, and PPAR signaling which are related to fat proliferation and differentiation [2].
In genetic studies, PLIN1 haploinsufficiency, rather than causing lipodystrophy as previously thought, leads to a favorable metabolic profile. Individuals with PLIN1 protein-truncating variants (PTVs) have increased high-density lipoprotein cholesterol, reduced triglycerides, waist-to-hip ratio, and systolic blood pressure, and a reduced risk of myocardial infarction and hypertension [1]. Also, in chicken preadipocytes, knockout of PLIN1 decreased differentiation ability and early apoptotic activity while increasing proliferation ability, and had differential effects on lipolysis under basal and hormone-stimulated conditions [3].
In conclusion, Plin1 is essential for lipid metabolism in adipocytes, regulating processes like lipolysis and fat storage. Studies with gene knockout models, especially in chicken preadipocytes, have provided insights into its role in cell proliferation, apoptosis, and differentiation. Additionally, understanding Plin1 haploinsufficiency has implications for cardiovascular disease prevention as it seems to confer a beneficial metabolic profile [1,3].
References:
1. Patel, Kashyap A, Burman, Shivang, Laver, Thomas W, Frayling, Timothy M, Weedon, Michael N. . PLIN1 Haploinsufficiency Causes a Favorable Metabolic Profile. In The Journal of clinical endocrinology and metabolism, 107, e2318-e2323. doi:10.1210/clinem/dgac104. https://pubmed.ncbi.nlm.nih.gov/35235652/
2. Li, Shijun, Raza, Sayed Haidar Abbas, Zhao, Chunping, Cheng, Gong, Zan, Linsen. 2020. Overexpression of PLIN1 Promotes Lipid Metabolism in Bovine Adipocytes. In Animals : an open access journal from MDPI, 10, . doi:10.3390/ani10111944. https://pubmed.ncbi.nlm.nih.gov/33105676/
3. Zhai, Guiying, Pang, Yongjia, Zou, Yichong, Li, Hui, Wang, Yuxiang. 2022. Effects of PLIN1 Gene Knockout on the Proliferation, Apoptosis, Differentiation and Lipolysis of Chicken Preadipocytes. In Animals : an open access journal from MDPI, 13, . doi:10.3390/ani13010092. https://pubmed.ncbi.nlm.nih.gov/36611701/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen