C57BL/6JCya-Psat1em1flox/Cya
Common Name:
Psat1-flox
Product ID:
S-CKO-00557
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Psat1-flox
Strain ID
CKOCMP-107272-Psat1-B6J-VA
Gene Name
Product ID
S-CKO-00557
Gene Alias
D8Ertd814e; EPIP; PSA; Psat
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Psat1em1flox/Cya mice (Catalog S-CKO-00557) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025542
NCBI RefSeq
NM_177420
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Psat1, also known as phosphoserine aminotransferase 1, is an enzyme in the serine biosynthesis pathway (SBP) [1-10]. The SBP is a branch of glycolysis, and Psat1 is one of its three key enzymes. It plays a crucial role in metabolism by producing metabolites like α-ketoglutarate (α-KG) and glutamine, which are involved in bioenergetics, biomass generation, and serving as cofactors for chromatin modifiers [1,7].
Gene inactivation studies in mice revealed that Psat1 controls muscle stem cell (MuSC) activation and expansion of myogenic progenitors [1]. Psat1 ablation led to defective MuSC expansion and impaired muscle regeneration [1].
Additionally, in tumors, targeting Psat1 has shown potential therapeutic effects. For example, in tumors with the p53-72Pro variant, depletion of PSAT1 restored the interaction between p5372P and PGC-1α, suppressing metastasis [2]. In triple-negative breast cancer, the miR-195-5p/PSAT1 feedback loop was identified, suggesting a potential targeted therapy [3]. In estrogen receptor-negative breast cancer, PSAT1 promoted metastasis via the p-AKT/SP1/ITGA2 axis [4]. In colorectal cancer, PSAT1 knockdown enhanced cell migration, invasion, proliferation, and the EMT process by regulating the PI3K/AKT signaling pathway [5]. In clear cell renal cell carcinoma, PSAT1 was associated with patient prognosis and was used to develop a new nomogram model for prognosis estimation [6].
In conclusion, Psat1 is essential for muscle stem cell activation and muscle regeneration [1]. In the context of diseases, especially cancer, studies on Psat1 gene knockout or knockdown models have provided insights into its role in tumor metastasis and prognosis, suggesting it could be a potential therapeutic target [2-6].
References:
1. Ciuffoli, Veronica, Feng, Xuesong, Jiang, Kan, Dell'Orso, Stefania, Sartorelli, Vittorio. 2024. Psat1-generated α-ketoglutarate and glutamine promote muscle stem cell activation and regeneration. In Genes & development, 38, 151-167. doi:10.1101/gad.351428.123. https://pubmed.ncbi.nlm.nih.gov/38453480/
2. Jiang, Jingwen, Chen, Hai-Ning, Jin, Ping, Yu, Qiang, Huang, Canhua. 2023. Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant. In Signal transduction and targeted therapy, 8, 65. doi:10.1038/s41392-022-01266-7. https://pubmed.ncbi.nlm.nih.gov/36788227/
3. Wang, Huiling, Fang, Qian, You, Shuo, Wu, Yaqin, Zhang, Chaojie. 2022. miRNA-195-5p/PSAT1 feedback loop in human triple-negative breast cancer cells. In Genes & genomics, 45, 39-47. doi:10.1007/s13258-022-01327-9. https://pubmed.ncbi.nlm.nih.gov/36371491/
4. Zhang, Xingda, Wang, Siyu, Li, Wei, Pang, Da, Gao, Song. 2024. PSAT1 Promotes Metastasis via p-AKT/SP1/ITGA2 Axis in Estrogen Receptor-Negative Breast Cancer Cell. In Biomolecules, 14, . doi:10.3390/biom14080990. https://pubmed.ncbi.nlm.nih.gov/39199378/
5. Wang, Mingjin, Zhang, Houshun, Lu, Zhiyuan, Wang, Jiayu, Jia, Xiaoyi. 2024. PSAT1 mediated EMT of colorectal cancer cells by regulating Pl3K/AKT signaling pathway. In Journal of Cancer, 15, 3183-3198. doi:10.7150/jca.93789. https://pubmed.ncbi.nlm.nih.gov/38706897/
6. Wang, Jun, He, Xiaoming, Mi, Yuanyuan, Li, Jie, Wang, Rong. 2024. PSAT1 enhances the efficacy of the prognosis estimation nomogram model in stage-based clear cell renal cell carcinoma. In BMC cancer, 24, 463. doi:10.1186/s12885-024-12183-z. https://pubmed.ncbi.nlm.nih.gov/38614981/
7. Qiu, Yijian, Stamatatos, Olivia T, Hu, Qingting, Cross, Justin R, Lukey, Michael J. 2024. The unique catalytic properties of PSAT1 mediate metabolic adaptation to glutamine blockade. In Nature metabolism, 6, 1529-1548. doi:10.1038/s42255-024-01104-w. https://pubmed.ncbi.nlm.nih.gov/39192144/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen