C57BL/6JCya-Fam72aem1flox/Cya
Common Name
Fam72a-flox
Product ID
S-CKO-00713
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-108900-Fam72a-B6J-VA
Status
When using this mouse strain in a publication, please cite “Fam72a-flox Mouse (Catalog S-CKO-00713) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Fam72a-flox
Strain ID
CKOCMP-108900-Fam72a-B6J-VA
Gene Name
Product ID
S-CKO-00713
Gene Alias
P17, 2700049P18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000068613
NCBI RefSeq
NM_175382
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Overview of Gene Research
Fam72a, a poorly characterized yet evolutionarily conserved gene across multicellular organisms, has emerged as a significant player in multiple biological processes. It is involved in cell-cycle regulation, interacting with key components of the cell-cycle machinery and apoptosis-related pathways. It also plays a crucial role in antibody maturation and mutagenic DNA repair pathways [2].
In Fam72a-deficient CH12F3-2 B cells and primary B cells from Fam72a-/-mice, reduced class-switch recombination and somatic hypermutation frequencies at immunoglobulin and Bcl6 genes were observed, along with reduced genome-wide deoxyuracils [1]. The somatic hypermutation spectrum in these cells was opposite to that in UNG2-deficient mice, suggesting hyperactivity of UNG2 in FAM72A-deficient cells. FAM72A binds to UNG2, reducing UNG2 protein levels in the G1 phase, leading to error-prone DNA repair [1]. In addition, Fam72a-/-B cells show defective class-switch recombination due to UNG2 upregulation, and reduced somatic hypermutation [3].
In conclusion, Fam72a is essential for error-prone DNA repair during antibody maturation by regulating UNG2 levels. The use of Fam72a-/-mouse models has revealed its significance in antibody-related biological processes. Moreover, its overexpression in many cancers may promote mutagenesis, indicating its potential importance in cancer research [1,3].
References：
1. Feng, Yuqing, Li, Conglei, Stewart, Jessica A, Bhagwat, Ashok S, Martin, Alberto. 2021. FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation. In Nature, 600, 324-328. doi:10.1038/s41586-021-04144-4. https://pubmed.ncbi.nlm.nih.gov/34819670/
2. Fu, Yuan, Jia, Xiaofan, Yuan, Jinwei, Zhou, Jun, Wang, Ting. 2023. Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates. In Developmental cell, 58, 398-415.e7. doi:10.1016/j.devcel.2023.02.006. https://pubmed.ncbi.nlm.nih.gov/36868233/
3. Rogier, Mélanie, Moritz, Jacques, Robert, Isabelle, Deriano, Ludovic, Reina-San-Martin, Bernardo. 2021. Fam72a enforces error-prone DNA repair during antibody diversification. In Nature, 600, 329-333. doi:10.1038/s41586-021-04093-y. https://pubmed.ncbi.nlm.nih.gov/34819671/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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