C57BL/6JCya-Mlecem1flox/Cya
Common Name:
Mlec-flox
Product ID:
S-CKO-00749
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mlec-flox
Strain ID
CKOCMP-109154-Mlec-B6J-VA
Gene Name
Product ID
S-CKO-00749
Gene Alias
2410014A08Rik; ESTM19; mKIAA0152
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mlecem1flox/Cya mice (Catalog S-CKO-00749) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112121
NCBI RefSeq
NM_175403
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
MLEC, also known as Malectin, is a glycoprotein quality control factor in the endoplasmic reticulum. It recognizes the Glc2Man9GlcNAc2 (G2M9) oligosaccharide chain on newly synthesized glycoproteins, playing a crucial role in glycoprotein quality control [3]. It has been implicated in multiple biological processes and diseases.
In the context of cerebral palsy, specific single nucleotide polymorphisms (SNPs) of MLEC gene were found to be associated with the pathogenesis. C alleles of rs10431386 and rs7964786 inhibit MLEC expression in the blood of CP patients and macrophage cell line, and in vitro studies suggest these alleles promote CP by inhibiting M1 to M2 macrophage polarization [1].
MLEC also promotes coronavirus replication and viral protein biogenesis, as knockdown of MLEC significantly reduces infectious titers, and during infection, it interacts with relevant proteins and promotes viral protein levels and genome replication [2].
Additionally, MLEC has been identified as a potential characteristic marker for papillary thyroid carcinoma, with immunohistochemistry confirming its overexpression in PTCs compared to normal surrounding tissues [4].
In conclusion, MLEC is important for glycoprotein quality control. Its gene polymorphisms are related to the pathogenesis of cerebral palsy, it promotes coronavirus replication, and may serve as a marker for papillary thyroid carcinoma. Studies on MLEC using genetic models have provided insights into these disease-related mechanisms, contributing to our understanding of the role of MLEC in specific biological processes and diseases.
References：
1. Shi, W, Zhu, Y, Zhou, M, Chen, X, Chen, X. 2018. Malectin gene polymorphisms promote cerebral palsy via M2-like macrophage polarization. In Clinical genetics, 93, 794-799. doi:10.1111/cge.13149. https://pubmed.ncbi.nlm.nih.gov/28972276/
2. Davies, Jonathan P, Plate, Lars. 2024. The glycoprotein quality control factor Malectin promotes coronavirus replication and viral protein biogenesis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.06.02.597051. https://pubmed.ncbi.nlm.nih.gov/38895409/
3. Qin, Sheng-Ying, Hu, Dan, Yamamoto, Kazuo. . Preparation of Soluble Malectin and Its Tetramer. In Methods in molecular biology (Clifton, N.J.), 2132, 285-294. doi:10.1007/978-1-0716-0430-4_28. https://pubmed.ncbi.nlm.nih.gov/32306336/
4. Ban, Yoshiyuki, Yamamoto, Gou, Takada, Michiya, Tachikawa, Tetsuhiko, Hirano, Tsutomu. 2012. Proteomic profiling of thyroid papillary carcinoma. In Journal of thyroid research, 2012, 815079. doi:10.1155/2012/815079. https://pubmed.ncbi.nlm.nih.gov/22518348/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen