C57BL/6JCya-Chaf1bem1flox/Cya
Common Name:
Chaf1b-flox
Product ID:
S-CKO-00883
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Chaf1b-flox
Strain ID
CKOCMP-110749-Chaf1b-B6J-VA
Gene Name
Product ID
S-CKO-00883
Gene Alias
2600017H24Rik; CAF-I p60; CAF-Ip60; CAF1; CAF1A; CAF1P60; MPHOSPH7
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chaf1bem1flox/Cya mice (Catalog S-CKO-00883) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023666
NCBI RefSeq
NM_028083
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Chaf1b, also known as chromatin assembly factor 1 subunit B or the p60 subunit of the chromatin-assembly factor-1 (CAF-1) complex, is crucial for nucleosome assembly following DNA synthesis, maintaining genomic stability [4]. It has multiple functional domains like the 7× WD repeat domain, B-like domain, and a PEST domain, but how these contribute to its functions is not fully clear. It is involved in various biological processes such as DNA synthesis and repair [3,6].
In mouse pre-implantation embryos, Chaf1b knockdown led to a low blastocyst hatching rate, outgrowth failure in vitro, and embryonic lethality in vivo. It increased apoptosis and down-regulated key cell fate specification regulators. Mechanistically, it mediated the replacement of H3.3 with H3.1/3.2, affecting histone marks and chromatin accessibility [2]. In myeloproliferative neoplasms (MPN), Chaf1b is overexpressed. Targeted silencing enhanced IFNα-stimulated gene transcription and promoted IFNα-dependent antineoplastic responses in primary MPN progenitor cells [1]. In nasopharyngeal carcinoma, high Chaf1b levels correlated with radioresistance. Silencing Chaf1b enhanced radiosensitivity by promoting apoptosis and inhibiting DNA damage repair in a DNA-PK pathway-dependent manner [3]. In leukemia, overexpression of Chaf1b displaced myeloid transcription factors from chromatin, while its deletion promoted leukemia cell differentiation and suppressed leukemogenesis in a murine model [5,7].
In summary, Chaf1b plays significant roles in embryo development, hematopoiesis, and cancer-related processes. Mouse models, especially gene knockout or conditional knockout models, have been instrumental in revealing its functions in these biological processes and disease conditions, highlighting its potential as a therapeutic target in diseases like MPN, nasopharyngeal carcinoma, and leukemia.
References:
1. Saleiro, Diana, Kosciuczuk, Ewa M, Fischietti, Mariafausta, Hoffman, Ronald, Platanias, Leonidas C. 2023. Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells. In Cancer research communications, 3, 943-951. doi:10.1158/2767-9764.CRC-23-0010. https://pubmed.ncbi.nlm.nih.gov/37377894/
2. Zhang, Yingbing, Yang, Ying, Qiao, Peipei, Zhang, Yong, Su, Jianmin. 2021. CHAF1b, chromatin assembly factor-1 subunit b, is essential for mouse preimplantation embryos. In International journal of biological macromolecules, 195, 547-557. doi:10.1016/j.ijbiomac.2021.11.181. https://pubmed.ncbi.nlm.nih.gov/34906611/
3. Di, Muping, Wang, Meng, Miao, Jingjing, Wang, Lin, Zhao, Chong. 2019. CHAF1B induces radioresistance by promoting DNA damage repair in nasopharyngeal carcinoma. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 123, 109748. doi:10.1016/j.biopha.2019.109748. https://pubmed.ncbi.nlm.nih.gov/31869663/
4. Volk, Andrew, Crispino, John D. 2015. The role of the chromatin assembly complex (CAF-1) and its p60 subunit (CHAF1b) in homeostasis and disease. In Biochimica et biophysica acta, 1849, 979-86. doi:10.1016/j.bbagrm.2015.05.009. https://pubmed.ncbi.nlm.nih.gov/26066981/
5. Li, Qing, Zhang, Xu, Zhang, Zhiguo. . CHAF1B Overexpression: A Brake for the Differentiation of Leukemia Cells. In Cancer cell, 34, 693-694. doi:10.1016/j.ccell.2018.10.011. https://pubmed.ncbi.nlm.nih.gov/30423290/
6. Duan, Yan, Liu, Tanzhen, Li, Shengwen, Zhao, Hui, Li, Jianqiang. 2019. CHAF1B promotes proliferation and reduces apoptosis in 95‑D lung cancer cells and predicts a poor prognosis in non‑small cell lung cancer. In Oncology reports, 41, 2518-2528. doi:10.3892/or.2019.6994. https://pubmed.ncbi.nlm.nih.gov/30720130/
7. Volk, Andrew, Liang, Kaiwei, Suraneni, Praveen, Shilatifard, Ali, Crispino, John D. . A CHAF1B-Dependent Molecular Switch in Hematopoiesis and Leukemia Pathogenesis. In Cancer cell, 34, 707-723.e7. doi:10.1016/j.ccell.2018.10.004. https://pubmed.ncbi.nlm.nih.gov/30423293/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen