C57BL/6JCya-Adamts1em1flox/Cya
Common Name:
Adamts1-flox
Product ID:
S-CKO-01052
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Adamts1-flox
Strain ID
CKOCMP-11504-Adamts1-B6J-VA
Gene Name
Product ID
S-CKO-01052
Gene Alias
ADAM-TS1; ADAMTS; ADAMTS-1; C3-C5; METH-1; METH1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adamts1em1flox/Cya mice (Catalog S-CKO-01052) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023610
NCBI RefSeq
NM_009621
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
ADAMTS1, also known as METH1, is a matrix metalloprotease. It has angioinhibitory properties, binds to VEGF and dampens VEGFR2 phosphorylation, with both its binding and catalytic domains likely required for antiangiogenic/antitumor activities [1]. It is also involved in many physiological and pathological conditions, such as tumorigenesis, metastasis, and ovulation [5,6].
In glioma, ADAMTS1 is highly expressed, correlated with malignancy grade and prognosis. Its knockout suppresses intracranial orthotopic xenograft growth and prolongs survival in mice, likely by affecting the Notch1-SOX2 signaling pathway in glioma stem cells [2].
In renal cell carcinoma, elevated ADAMTS1 is associated with poor prognosis. Manipulating ADAMTS1 affects cell anoikis through the mitochondrial pathway. A cyclic axis of ADAMTS1-VCAM-EGFR drives anoikis resistance and invasion [3].
In non-small cell lung cancer, ADAMTS1 promotes cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) by regulating TGF-β [4].
In polycystic kidney disease, loss of Pkd1 increases Adamts1 mRNA expression, leading to versican cleavage, macrophage accumulation, and cyst growth. Deletion of both Adamts1 and Pkd1 reduces these effects and improves kidney function [7].
In conclusion, ADAMTS1 plays diverse and significant roles in multiple biological processes and diseases. Through gene knockout models in mice, its functions in tumorigenesis, metastasis, and cyst expansion in polycystic kidney disease have been well-demonstrated, providing valuable insights into the underlying mechanisms and potential therapeutic targets for these diseases.
References:
1. Iruela-Arispe, M Luisa, Carpizo, Darren, Luque, Alfonso. . ADAMTS1: a matrix metalloprotease with angioinhibitory properties. In Annals of the New York Academy of Sciences, 995, 183-90. doi:. https://pubmed.ncbi.nlm.nih.gov/12814950/
2. Wang, Shanshan, Zhang, Jin, Wang, Ke, Zhao, Yuanli, Liu, Dongying. 2022. ADAMTS1 as potential prognostic biomarker promotes malignant invasion of glioma. In International journal of clinical oncology, 28, 52-68. doi:10.1007/s10147-022-02268-9. https://pubmed.ncbi.nlm.nih.gov/36371587/
3. Wen, Yu-Ching, Lin, Yung-Wei, Ho, Kuo-Hao, Lee, Wei-Jiunn, Chien, Ming-Hsien. 2024. The oncogenic ADAMTS1-VCAN-EGFR cyclic axis drives anoikis resistance and invasion in renal cell carcinoma. In Cellular & molecular biology letters, 29, 126. doi:10.1186/s11658-024-00643-0. https://pubmed.ncbi.nlm.nih.gov/39333870/
4. Hu, Xueqian, Jiang, Chunqi, Hu, Ning, Hong, Shanyi. 2023. ADAMTS1 induces epithelial-mesenchymal transition pathway in non-small cell lung cancer by regulating TGF-β. In Aging, 15, 2097-2114. doi:10.18632/aging.204594. https://pubmed.ncbi.nlm.nih.gov/36947712/
5. Tan, Izza de Arao, Ricciardelli, Carmela, Russell, Darryl L. 2013. The metalloproteinase ADAMTS1: a comprehensive review of its role in tumorigenic and metastatic pathways. In International journal of cancer, 133, 2263-76. doi:10.1002/ijc.28127. https://pubmed.ncbi.nlm.nih.gov/23444028/
6. Baker, Sheridan J C, Van Der Kraak, Glen. 2021. ADAMTS1 is regulated by the EP4 receptor in the zebrafish ovary. In General and comparative endocrinology, 311, 113835. doi:10.1016/j.ygcen.2021.113835. https://pubmed.ncbi.nlm.nih.gov/34181931/
7. Kakade, Vijayakumar R, Akman, Zafer, Motrapu, Manga, Somlo, Stefan, Cantley, Lloyd G. 2024. Adamts1 and Cyst Expansion in Polycystic Kidney Disease. In Journal of the American Society of Nephrology : JASN, 36, 559-570. doi:10.1681/ASN.0000000557. https://pubmed.ncbi.nlm.nih.gov/39514301/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen