C57BL/6JCya-Car11em1flox/Cya
Common Name:
Car11-flox
Product ID:
S-CKO-01535
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Car11-flox
Strain ID
CKOCMP-12348-Car11-B6J-VA
Gene Name
Product ID
S-CKO-01535
Gene Alias
Ca11
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Car11em1flox/Cya mice (Catalog S-CKO-01535) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003360
NCBI RefSeq
NM_009800
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Car11, also known as carbonic anhydrase-related protein XI (CA-RP XI), is a member of the alpha-carbonic anhydrase gene family. Although it lacks the typical catalytic activity of carbonic anhydrases due to inactivating changes in active-site residues, it likely has an important biological function. It may be involved in intracellular signal transduction as its amino-and carboxy-terminal regions have potential phosphorylation sites and binding motifs [5].
Transcriptional profiling detected Car11 expression in the neonatal mouse uterus between Postnatal Days 3 and 18, where its mRNA was predominantly localized in endometrial stromal cells [1]. In studies of Alzheimer's disease-related mouse models (5XFAD and T2KO mice), differential transcript usage (DTU) in Car11 was observed in neuronal and glial subtypes compared to wild-type mice, highlighting its potential role in the pathophysiology of Alzheimer's disease [2]. Phylogenetic analysis showed that the duplication of CA10 into Car11 occurred only in tetrapods. Immunohistochemical analysis and real-time quantitative PCR in mice indicated a wide distribution of Car11 mRNA, with positive signals in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis [3]. During mammalian inner ear development, Car11 transcripts were expressed at higher levels, suggesting a role in inner ear development and function [4]. In murine embryonic studies, Car11 message was seen at an early gestational stage and expressed to a lesser extent as gestation progressed, and it was expressed in neuroprogenitor cells in the subventricular area and neural cells migrating to the cortex, suggesting an important role in the central nervous system (CNS) [6].
In conclusion, Car11 has a wide tissue distribution and is involved in multiple biological processes such as uterine development, inner ear development, and potentially in the pathophysiology of Alzheimer's disease and in CNS development. The use of mouse models in these studies has been crucial in uncovering these roles, providing insights into its functions and implications in various biological and disease-related contexts.
References:
1. Hu, Jianbo, Spencer, Thomas E. 2005. Carbonic anhydrase regulate endometrial gland development in the neonatal uterus. In Biology of reproduction, 73, 131-8. doi:. https://pubmed.ncbi.nlm.nih.gov/15788756/
2. Weller, Andrew E, Ferraro, Thomas N, Doyle, Glenn A, Crist, Richard C, Berrettini, Wade H. . Single Nucleus Transcriptome Data from Alzheimer's Disease Mouse Models Yield New Insight into Pathophysiology. In Journal of Alzheimer's disease : JAD, 90, 1233-1247. doi:10.3233/JAD-220391. https://pubmed.ncbi.nlm.nih.gov/36213995/
3. Aspatwar, Ashok, Tolvanen, Martti Ee, Parkkila, Seppo. 2010. Phylogeny and expression of carbonic anhydrase-related proteins. In BMC molecular biology, 11, 25. doi:10.1186/1471-2199-11-25. https://pubmed.ncbi.nlm.nih.gov/20356370/
4. Wu, Ling, Sagong, Borum, Choi, Jae Young, Kim, Un-Kyung, Bok, Jinwoong. 2013. A systematic survey of carbonic anhydrase mRNA expression during mammalian inner ear development. In Developmental dynamics : an official publication of the American Association of Anatomists, 242, 269-80. doi:10.1002/dvdy.23917. https://pubmed.ncbi.nlm.nih.gov/23233153/
5. Lovejoy, D A, Hewett-Emmett, D, Porter, C A, Vale, W W, Tashian, R E. . Evolutionarily conserved, "acatalytic" carbonic anhydrase-related protein XI contains a sequence motif present in the neuropeptide sauvagine: the human CA-RP XI gene (CA11) is embedded between the secretor gene cluster and the DBP gene at 19q13.3. In Genomics, 54, 484-93. doi:. https://pubmed.ncbi.nlm.nih.gov/9878252/
6. Taniuchi, Keisuke, Nishimori, Isao, Takeuchi, Tamotsu, Ohtsuki, Yuji, Onishi, Saburo. . cDNA cloning and developmental expression of murine carbonic anhydrase-related proteins VIII, X, and XI. In Brain research. Molecular brain research, 109, 207-15. doi:. https://pubmed.ncbi.nlm.nih.gov/12531530/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen