C57BL/6JCya-Car2em1flox/Cya
Common Name:
Car2-flox
Product ID:
S-CKO-01536
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Car2-flox
Strain ID
CKOCMP-12349-Car2-B6J-VA
Gene Name
Product ID
S-CKO-01536
Gene Alias
CAII; Ca2; Car-2; Ltw-5; Lvtw-5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Car2em1flox/Cya mice (Catalog S-CKO-01536) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029078
NCBI RefSeq
NM_009801
Target Region
Exon 3~4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Car2, also known as carbonic anhydrase 2, encodes the primary isoenzyme responsible for aqueous humor (AH) production and plays a major role in the regulation of intraocular pressure (IOP). It is involved in the physiological process related to eye pressure regulation [1].
In a study, CRISPR-Cas9-mediated deletion of Car2 in the ciliary body of mice was carried out. With a single intravitreal injection, Car2 knockout significantly and sustainably reduced IOP in both normal mice and glaucoma models by inhibiting AH production. It also effectively delayed and even halted glaucomatous damage induced by prolonged high IOP in a chronic ocular hypertension model, outperforming clinically available carbonic anhydrase inhibitors [1].
In conclusion, Car2 is crucial for the regulation of intraocular pressure. The Car2 knockout mouse model has revealed its potential as a therapeutic target for glaucoma. The findings suggest that targeting Car2 could be an attractive therapeutic strategy for patients with glaucoma [1].
References:
1. Jiang, Jiaxuan, Kong, Kangjie, Fang, Xiuli, Yu-Wai-Man, Patrick, Zhang, Xiulan. 2024. CRISPR-Cas9-mediated deletion of carbonic anhydrase 2 in the ciliary body to treat glaucoma. In Cell reports. Medicine, 5, 101524. doi:10.1016/j.xcrm.2024.101524. https://pubmed.ncbi.nlm.nih.gov/38670096/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen