C57BL/6JCya-Casrem1flox/Cya
Common Name:
Casr-flox
Product ID:
S-CKO-01556
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Casr-flox
Strain ID
CKOCMP-12374-Casr-B6J-VA
Gene Name
Product ID
S-CKO-01556
Gene Alias
CaR; Gprc2a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Casrem1flox/Cya mice (Catalog S-CKO-01556) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063597
NCBI RefSeq
NM_013803
Target Region
Exon 3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
CaSR, the calcium-sensing receptor, is a class-C G protein-coupled receptor. It plays a pivotal role in regulating calcium homeostasis by sensing extracellular Ca2+ concentration changes. It is also involved in various other cellular processes such as gene transcription, cell proliferation, differentiation, apoptosis, muscle contraction, and neuronal transmission. The CaSR-mediated intracellular Ca2+ signaling is part of key biological pathways [2].
In conditional and global CaSR knockout mice, colonic epithelia showed increased proliferation with enhanced Wnt/β-catenin signaling, indicating that CaSR negatively modulates colonic epithelial growth [3]. In breast cancer, CaSR acts as an oncoprotein and drives the pathogenesis of skeletal metastases. Understanding the CaSR signaling in this context could lead to novel therapeutic interventions for metastatic breast cancer [1,5]. Mutations in the CASR gene cause autosomal dominant hypocalcemia type 1 (ADH1), characterized by hypocalcemia, low parathyroid hormone levels, and high urinary calcium [4].
In conclusion, CaSR is crucial for calcium homeostasis and various cellular functions. Gene knockout mouse models have revealed its role in colonic epithelial growth regulation and in the pathogenesis of breast cancer bone metastases and ADH1. Research on CaSR provides insights into the molecular basis of these disease conditions and may facilitate the development of targeted therapeutic agents.
References:
1. Das, Souvik, Clézardin, Philippe, Kamel, Said, Brazier, Michel, Mentaverri, Romuald. 2020. The CaSR in Pathogenesis of Breast Cancer: A New Target for Early Stage Bone Metastases. In Frontiers in oncology, 10, 69. doi:10.3389/fonc.2020.00069. https://pubmed.ncbi.nlm.nih.gov/32117726/
2. Tian, Li, Andrews, Corey, Yan, Qiuyun, Yang, Jenny J. 2024. Molecular regulation of calcium-sensing receptor (CaSR)-mediated signaling. In Chronic diseases and translational medicine, 10, 167-194. doi:10.1002/cdt3.123. https://pubmed.ncbi.nlm.nih.gov/39027195/
3. Macleod, R John. 2013. CaSR function in the intestine: Hormone secretion, electrolyte absorption and secretion, paracrine non-canonical Wnt signaling and colonic crypt cell proliferation. In Best practice & research. Clinical endocrinology & metabolism, 27, 385-402. doi:10.1016/j.beem.2013.05.005. https://pubmed.ncbi.nlm.nih.gov/23856267/
4. Wu, Yingying, Zhang, Chao, Huang, Xiaojun, Liu, Shihua, Zhong, Ping. . Autosomal dominant hypocalcemia with a novel CASR mutation: a case study and literature review. In The Journal of international medical research, 50, 3000605221110489. doi:10.1177/03000605221110489. https://pubmed.ncbi.nlm.nih.gov/35818129/
5. Yang, Yanmei, Wang, Bin. 2018. PTH1R-CaSR Cross Talk: New Treatment Options for Breast Cancer Osteolytic Bone Metastases. In International journal of endocrinology, 2018, 7120979. doi:10.1155/2018/7120979. https://pubmed.ncbi.nlm.nih.gov/30151009/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen