C57BL/6JCya-Cbx5em1flox/Cya
Common Name
Cbx5-flox
Product ID
S-CKO-01580
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12419-Cbx5-B6J-VA
Status
When using this mouse strain in a publication, please cite “Cbx5-flox Mouse (Catalog S-CKO-01580) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Cbx5-flox
Strain ID
CKOCMP-12419-Cbx5-B6J-VA
Gene Name
Product ID
S-CKO-01580
Gene Alias
HP1, Hp1a, Hp1alpha, 2610029O15Rik
Background
C57BL/6JCya
Gene Full Name
chromobox 5
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 15 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000118152
NCBI Transcript ID
NM_001076789
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Overview of Gene Research
CBX5, also known as HP1α, is an epigenetic regulatory protein. It is a methyl reader that interprets H3K9me3 marks mediated by H3K9 methyltransferases, contributing to the formation of inaccessible heterochromatin and suppression of gene expression. CBX5 is involved in multiple biological processes, including maintaining chromatin state, and is associated with pathways relevant to cell growth, apoptosis, and fibroblast activation [1,4,5].
In myeloid leukemia, loss of RBMX/L1, which control the transcription of CBX5, led to changes in chromatin accessibility and leukemia development was delayed. Forced CBX5 expression rescued the effects of RBMX/L1 depletion on cell growth and apoptosis, indicating CBX5 is crucial for leukemia cell survival [1].
In lung cancer, CBX5 loss drives EGFR inhibitor resistance. CBX5-knockdown in EGFR-mutant lung adenocarcinoma cells increased E2F1 expression, which upregulated the anti-apoptotic gene BIRC5, attenuating apoptosis induction by EGFR inhibitors [2].
In periodontitis, Porphyromonas gingivalis OMVs' sRNA45033 targets CBX5, which regulates apoptosis through p53 DNA methylation [3].
In lung fibrosis, the CBX5/G9a/H3K9me-mediated gene repression is essential for fibroblast activation. Inhibition of this pathway elevates PGC1α, attenuating fibrosis [4].
In summary, CBX5 is vital for maintaining chromatin state, cell growth, apoptosis regulation, and fibroblast activation. Research using gene-knockout or related models in myeloid leukemia, lung cancer, periodontitis, and lung fibrosis has revealed its significant roles in these disease conditions, highlighting its potential as a therapeutic target in these areas.
References：
1. Prieto, Camila, Nguyen, Diu T T, Liu, Zhaoqi, Rabadan, Raul, Kharas, Michael G. 2021. Transcriptional control of CBX5 by the RNA binding proteins RBMX and RBMXL1 maintains chromatin state in myeloid leukemia. In Nature cancer, 2, 741-757. doi:10.1038/s43018-021-00220-w. https://pubmed.ncbi.nlm.nih.gov/34458856/
2. Bugide, Suresh, Edwards, Yvonne J K, Gupta, Romi, Green, Michael R, Wajapeyee, Narendra. 2023. CBX5 loss drives EGFR inhibitor resistance and results in therapeutically actionable vulnerabilities in lung cancer. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2218118120. doi:10.1073/pnas.2218118120. https://pubmed.ncbi.nlm.nih.gov/36652476/
3. Fan, Ruyi, Zhou, Yi, Chen, Xu, Wang, Xiaoqian, Xu, Yan. 2023. Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis. In Microbiology spectrum, 11, e0328822. doi:10.1128/spectrum.03288-22. https://pubmed.ncbi.nlm.nih.gov/36629433/
4. Ligresti, Giovanni, Caporarello, Nunzia, Meridew, Jeffrey A, Urrutia, Raul A, Tschumperlin, Daniel J. 2019. CBX5/G9a/H3K9me-mediated gene repression is essential to fibroblast activation during lung fibrosis. In JCI insight, 5, . doi:10.1172/jci.insight.127111. https://pubmed.ncbi.nlm.nih.gov/31095524/
5. van Wijnen, Andre J, Bagheri, Leila, Badreldin, Amr A, Paradise, Christopher R, Wu, Zhong. 2020. Biological functions of chromobox (CBX) proteins in stem cell self-renewal, lineage-commitment, cancer and development. In Bone, 143, 115659. doi:10.1016/j.bone.2020.115659. https://pubmed.ncbi.nlm.nih.gov/32979540/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
