C57BL/6JCya-Crabp1em1flox/Cya
Common Name:
Crabp1-flox
Product ID:
S-CKO-01852
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Crabp1-flox
Strain ID
CKOCMP-12903-Crabp1-B6J-VA
Gene Name
Product ID
S-CKO-01852
Gene Alias
CRABP-I; Crabp-1; CrabpI; Rbp-5
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crabp1em1flox/Cya mice (Catalog S-CKO-01852) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034830
NCBI RefSeq
NM_013496
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Cellular Retinoic Acid Binding Protein 1 (CRABP1) is a mediator of non-canonical activities of retinoic acid (RA). It forms complexes with specific signaling molecules, functioning as RA-regulated signalsomes in a cell-context-dependent manner. CRABP1 is involved in multiple pathways, such as regulating MAPK and CaMKII activities, which are associated with stem cell proliferation, cancers, adipocyte health, neuro-immune regulation, heart and motor neuron diseases [1].
Crabp1 knockout (CKO) mouse models have been instrumental in revealing its functions. CKO mice exhibit an adult-onset ALS-like phenotype with deteriorated neuromuscular junctions, indicating CRABP1's role in maintaining neuromuscular junctions through the CRABP1-CaMKII-Agrn signaling pathway [3]. They also show altered exosome profiles, suggesting a novel function of CRABP1 in modulating exosome secretion [2]. Additionally, CKO mice have reduced anxiety-like behaviors, altered HPA axis feedback inhibition due to changes in FKBP5 expression [4], and develop hypertrophy with elevated CaMKII phosphorylation, making them more susceptible to isoproterenol-induced cardiac injury [6]. Ablation of ARCCRABP1 neurons (neurons expressing CRABP1 in the arcuate nucleus) in mice leads to obesity and a diabetic phenotype, demonstrating their role in energy homeostasis regulation [5].
In conclusion, CRABP1 is crucial for multiple biological processes. Studies using CKO mouse models have revealed its significance in diseases like ALS, heart diseases, and stress-related disorders, as well as in physiological functions such as exosome secretion and energy homeostasis regulation. These findings provide valuable insights into the mechanisms underlying these conditions and potential therapeutic targets.
References:
1. Nhieu, Jennifer, Lin, Yu-Lung, Wei, Li-Na. 2022. CRABP1 in Non-Canonical Activities of Retinoic Acid in Health and Diseases. In Nutrients, 14, . doi:10.3390/nu14071528. https://pubmed.ncbi.nlm.nih.gov/35406141/
2. Nhieu, Jennifer, Wei, Chin-Wen, Ludwig, Megan, Drake, Justin M, Wei, Li-Na. 2024. CRABP1-complexes in exosome secretion. In Cell communication and signaling : CCS, 22, 381. doi:10.1186/s12964-024-01749-w. https://pubmed.ncbi.nlm.nih.gov/39075476/
3. Lin, Yu-Lung, Nhieu, Jennifer, Liu, Pei-Yao, Lowe, Dawn, Wei, Li-Na. 2022. CRABP1-CaMKII-Agrn regulates the maintenance of neuromuscular junction in spinal motor neuron. In Cell death and differentiation, 29, 1744-1756. doi:10.1038/s41418-022-00959-4. https://pubmed.ncbi.nlm.nih.gov/35217789/
4. Lin, Yu-Lung, Wei, Chin-Wen, Lerdall, Thomas A, Nhieu, Jennifer, Wei, Li-Na. 2021. Crabp1 Modulates HPA Axis Homeostasis and Anxiety-like Behaviors by Altering FKBP5 Expression. In International journal of molecular sciences, 22, . doi:10.3390/ijms222212240. https://pubmed.ncbi.nlm.nih.gov/34830120/
5. Yan, Lihong, Zhang, Xin, Jin, Liling, Liu, An, Li, Juxue. 2025. The ARCCRABP1 neurons play a crucial role in the regulation of energy homeostasis. In Nature communications, 16, 2319. doi:10.1038/s41467-025-57411-7. https://pubmed.ncbi.nlm.nih.gov/40057489/
6. Park, Sung Wook, Persaud, Shawna D, Ogokeh, Stanislas, Townsend, DeWayne, Wei, Li-Na. 2018. CRABP1 protects the heart from isoproterenol-induced acute and chronic remodeling. In The Journal of endocrinology, 236, 151-165. doi:10.1530/JOE-17-0613. https://pubmed.ncbi.nlm.nih.gov/29371236/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen