C57BL/6JCya-Csf3em1flox/Cya
Common Name:
Csf3-flox
Product ID:
S-CKO-01908
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Csf3-flox
Strain ID
CKOCMP-12985-Csf3-B6J-VA
Gene Name
Product ID
S-CKO-01908
Gene Alias
Csfg; G-CSF; MGI-IG
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Csf3em1flox/Cya mice (Catalog S-CKO-01908) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038886
NCBI RefSeq
NM_009971
Target Region
Exon 1~5
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Csf3, also known as colony-stimulating factor 3 or G-CSF, is a key cytokine in neutrophil production and function. It stimulates the maturation, proliferation, and trafficking of myeloid progenitor cells, and is involved in cytokine-cytokine receptor interaction pathways [1,3]. Its biological importance lies in its role in maintaining normal neutrophil levels and immune responses. Genetic models, such as gene knockout mouse models, have been crucial in understanding its functions.
In humans, biallelic inactivating variants in Csf3 have been found to cause a novel autosomal recessive form of severe congenital neutropenia (SCN), mirroring the phenotype observed in mouse and zebrafish models of Csf3 deficiency [1]. In mouse models of acute exacerbation of pulmonary fibrosis (AE-PF), exogenous Csf3 protein exacerbates the condition by disrupting alveolar epithelial barrier integrity via the PI3K/p-Akt/Snail pathway [2].
In conclusion, Csf3 is essential for neutrophil-related biological functions. The study of Csf3 knockout mouse models has revealed its significant role in diseases like SCN and AE-PF, enhancing our understanding of the underlying mechanisms and potentially guiding future therapeutic strategies for these conditions.
References:
1. Khouj, Ebtissal, Marafi, Dana, Aljamal, Bayan, Awaji, Ali, Alkuraya, Fowzan S. 2023. Human 'knockouts' of CSF3 display severe congenital neutropenia. In British journal of haematology, 203, 477-480. doi:10.1111/bjh.19054. https://pubmed.ncbi.nlm.nih.gov/37612131/
2. Guo, Bingnan, Liu, Wenwen, Ji, Xuan, Yan, Xianliang, Chen, Bi. 2024. CSF3 aggravates acute exacerbation of pulmonary fibrosis by disrupting alveolar epithelial barrier integrity. In International immunopharmacology, 135, 112322. doi:10.1016/j.intimp.2024.112322. https://pubmed.ncbi.nlm.nih.gov/38788452/
3. Xu, Junfeng, Li, Na, Xie, Hui, Yang, Lang, Jin, Peng. 2025. CSF3 promotes colorectal cancer progression by activating p65/NF-κB signaling pathway and inducing an immunosuppressive microenvironment. In Translational oncology, 53, 102310. doi:10.1016/j.tranon.2025.102310. https://pubmed.ncbi.nlm.nih.gov/39929064/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen