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C57BL/6JCya-Dazlem1flox/Cya
Common Name:
Dazl-flox
Product ID:
S-CKO-01996
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dazl-flox
Strain ID
CKOCMP-13164-Dazl-B6J-VA
Gene Name
Dazl
Product ID
S-CKO-01996
Gene Alias
Daz-like; Dazh; Dazl1; Dazla; Tpx-2; Tpx2
Background
C57BL/6JCya
NCBI ID
13164
Modification
Conditional knockout
Chromosome
17
Phenotype
MGI:1342328
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dazlem1flox/Cya mice (Catalog S-CKO-01996) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000010736
NCBI RefSeq
NM_010021
Target Region
Exon 4~6
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
DAZL, short for Deleted in Azoospermia-Like, is a germ cell-specific RNA-binding protein. It plays a crucial role in gametogenesis, regulating the translation and localization of specific messenger RNAs (mRNAs) during early development and gametogenesis [2]. It binds to GUU triplets in the 3' untranslated region of target mRNAs and is involved in translational regulation, which is essential for the normal development and function of germ cells [2].

In gene knockout studies, postnatal stage-specific deletion of Dazl in mouse germ cells led to complete male sterility with loss of spermatogonial stem cells, meiotic arrest, and spermatid arrest, while female fertility was not affected. DAZL was found to bind to a large number of testicular mRNA transcripts at the 3'-untranslated region and interact with translation proteins, and its absence led to a decrease in polysome-associated target transcripts and spermatogenic proteins, causing developmental arrest [4]. In DAZL-KO pigs, complete germ cell depletion was observed by 10 weeks old, and after spermatogonial stem cell transplantation, they could support donor-derived spermatogenesis, but with low spermatogenic efficiency [1]. In chicken primordial germ cells (PGCs), loss of DAZL expression led to decreased proliferation and survival, and DAZL was found to control genes related to germline development, pluripotency, and cell proliferation [3].

In conclusion, DAZL is a master regulator essential for spermatogenesis and germ cell survival, as revealed through gene knockout models in mice, pigs, and chickens. Its deletion or dysfunction can lead to male infertility, highlighting its importance in understanding the mechanisms of male reproductive diseases [1,3,4].

References:
1. Lara, Nathalia L M, Goldsmith, Taylor, Rodriguez-Villamil, Paula, Carlson, Daniel F, Dobrinski, Ina. 2023. DAZL Knockout Pigs as Recipients for Spermatogonial Stem Cell Transplantation. In Cells, 12, . doi:10.3390/cells12212582. https://pubmed.ncbi.nlm.nih.gov/37947660/
2. Smorag, Lukasz, Xu, Xingbo, Engel, Wolfgang, Pantakani, D V Krishna. 2014. The roles of DAZL in RNA biology and development. In Wiley interdisciplinary reviews. RNA, 5, 527-35. doi:10.1002/wrna.1228. https://pubmed.ncbi.nlm.nih.gov/24715697/
3. Huang, Zhenwen, Xie, Long, Feng, Hu, Pu, Liping, Lu, Yangqing. 2024. DAZL regulate germline, pluripotency, and proliferation related genes in chicken PGCs and cooperate with DDX4. In Theriogenology, 222, 22-30. doi:10.1016/j.theriogenology.2024.03.010. https://pubmed.ncbi.nlm.nih.gov/38615433/
4. Li, Haixin, Liang, Zhuqing, Yang, Jian, Geng, Baobao, Xu, Eugene Yujun. 2018. DAZL is a master translational regulator of murine spermatogenesis. In National science review, 6, 455-468. doi:10.1093/nsr/nwy163. https://pubmed.ncbi.nlm.nih.gov/31355046/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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