C57BL/6JCya-Ddcem1flox/Cya
Common Name:
Ddc-flox
Product ID:
S-CKO-02014
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ddc-flox
Strain ID
CKOCMP-13195-Ddc-B6J-VA
Gene Name
Product ID
S-CKO-02014
Gene Alias
Aadc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ddcem1flox/Cya mice (Catalog S-CKO-02014) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000178704
NCBI RefSeq
NM_001190448
Target Region
Exon 7
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Ddc, also known as aromatic-l-amino-acid decarboxylase (AADC), is an enzyme that converts L-Dopa to dopamine [2]. Dopamine is a significant signaling molecule in the neuroendocrine system [3]. Ddc is thus crucial for dopamine synthesis, which is involved in numerous biological processes including neurotransmission, and is of great biological importance. Genetic models can be valuable for studying its functions.
In Parkinson's disease research, due to the relative sparing of Ddc expression while tyrosine hydroxylase (TH) expression is reduced in the substantia nigra pars compacta (SNpc), Ddc-positive neurons may be a therapeutic target. An AAV vector with a rat Ddc promoter was developed to target Ddc-positive neurons in the SNpc. Chemogenetic activation of these neurons in an A53T-mutated alpha-synuclein overexpression model of PD alleviated parkinsonian motor symptoms and rescued nigrostriatal TH expression, suggesting a potential therapeutic strategy for PD [1]. Also, in Procambarus clarkii, knocking down Pc-ddc led to changes in dopamine receptor (Pc-dar) expression, endogenous Pc-DDC enzymatic activities, and activation of the Toll (TLRs) signaling pathway and ROS-related antioxidant enzyme genes after bacterial challenge, indicating that Ddc and its related receptor play mediating roles in the antibacterial innate immune reactions of the hepatopancreas [3].
In conclusion, Ddc is essential for dopamine synthesis, playing key roles in neurotransmission and the neuroendocrine-immune system. In the context of Parkinson's disease, Ddc-positive neurons could potentially be a therapeutic target. The findings from model-based research, such as the use of A53T-mutated alpha-synuclein overexpression model in PD and RNAi assay in Procambarus clarkii, have provided insights into Ddc's functions in disease conditions and biological processes.
References:
1. Seo, Dong-Chan, Ju, Yeon Ha, Seo, Jin-Ju, Lee, Seung Eun, Nam, Min-Ho. 2023. DDC-Promoter-Driven Chemogenetic Activation of SNpc Dopaminergic Neurons Alleviates Parkinsonian Motor Symptoms. In International journal of molecular sciences, 24, . doi:10.3390/ijms24032491. https://pubmed.ncbi.nlm.nih.gov/36768816/
2. Lotsios, Nikolaos S, Arvanitis, Nikolaos, Charonitakis, Alexandros G, Sideris, Diamantis C, Vassilacopoulou, Dido. 2023. Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative Stress. In Current issues in molecular biology, 45, 10179-10192. doi:10.3390/cimb45120635. https://pubmed.ncbi.nlm.nih.gov/38132481/
3. Lin, Sihan, Wang, Kai, Yang, Bingbing, Shen, Xiuli, Du, Zhiqiang. 2022. Dopamine receptor (DAR) and dopa decarboxylase (DDC) mediate hepatopancreas antibacterial innate immune reactions in Procambarus clarkii. In International journal of biological macromolecules, 214, 140-151. doi:10.1016/j.ijbiomac.2022.05.200. https://pubmed.ncbi.nlm.nih.gov/35679960/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen