C57BL/6JCya-Adgre1em1flox/Cya
Common Name:
Adgre1-flox
Product ID:
S-CKO-02196
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Adgre1-flox
Strain ID
CKOCMP-13733-Adgre1-B6J-VA
Gene Name
Product ID
S-CKO-02196
Gene Alias
DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adgre1em1flox/Cya mice (Catalog S-CKO-02196) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086763
NCBI RefSeq
NM_010130
Target Region
Exon 10
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Adgre1, also known as EMR1 and F4/80, is an adhesion G protein-coupled receptor. It is widely used as a monocyte-macrophage marker in mice. ADGRE1 contains repeated Epidermal Growth Factor (EGF)-like calcium-binding domains in its extracellular domain. Its expression is induced by macrophage colony-stimulating factor (CSF1) treatment in vivo, and it may play a role in pathogen recognition due to its rapid evolution [1,2].
In colon cancer, tumor-associated macrophages (TAMs) can upregulate EMR1 (ADGRE1) in cancer cells. This upregulation promotes colon cancer progression by activating the JAK2/STAT1,3 signaling pathway, increasing cell proliferation, migration, and motility, and reducing apoptosis. Inhibition of JAK2/STAT3 can significantly impede the proliferation, migration, and mobility of cells and increase the apoptosis of EMR1+ colon cancer cells. Also, EMR1 expression in colon cancer cells is related to M2 macrophage polarization, and CRC with a high combined EMR1-TC+CD68+CD163+ score shows a worse recurrence-free survival prognosis [3,4].
In conclusion, Adgre1 is a key marker for monocyte-macrophages with potential roles in pathogen recognition. In colon cancer, the upregulation of Adgre1 by TAMs is involved in cancer progression through the JAK2/STAT1,3 signaling pathway, highlighting its significance in understanding colon cancer development and potentially providing new therapeutic targets.
References：
1. Hamann, Jörg, Aust, Gabriela, Araç, Demet, Langenhan, Tobias, Schiöth, Helgi B. . International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors. In Pharmacological reviews, 67, 338-67. doi:10.1124/pr.114.009647. https://pubmed.ncbi.nlm.nih.gov/25713288/
2. Waddell, Lindsey A, Lefevre, Lucas, Bush, Stephen J, Hope, Jayne C, Hume, David A. 2018. ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene Expressed in Mammalian Monocyte-Macrophages. In Frontiers in immunology, 9, 2246. doi:10.3389/fimmu.2018.02246. https://pubmed.ncbi.nlm.nih.gov/30327653/
3. Akter, Rokeya, Park, Rackhyun, Lee, Soo Kyung, Park, Junsoo, Cho, Mee-Yon. 2024. Upregulation of EMR1 (ADGRE1) by Tumor-Associated Macrophages Promotes Colon Cancer Progression by Activating the JAK2/STAT1,3 Signaling Pathway in Tumor Cells. In International journal of molecular sciences, 25, . doi:10.3390/ijms25084388. https://pubmed.ncbi.nlm.nih.gov/38673975/
4. Akter, Rokeya, Kim, Kwangmin, Kwon, Hye Youn, Cho, Hye-Mi, Cho, Mee-Yon. 2022. EMR1/ADGRE1 Expression in Cancer Cells Upregulated by Tumor-Associated Macrophages Is Related to Poor Prognosis in Colorectal Cancer. In Biomedicines, 10, . doi:10.3390/biomedicines10123121. https://pubmed.ncbi.nlm.nih.gov/36551877/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen