C57BL/6NCya-Gfi1em1flox/Cya
Common Name:
Gfi1-flox
Product ID:
S-CKO-02611
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gfi1-flox
Strain ID
CKOCMP-14581-Gfi1-B6N-VA
Gene Name
Product ID
S-CKO-02611
Gene Alias
Gfi-1; Pal-1; Pal1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Gfi1em1flox/Cya mice (Catalog S-CKO-02611) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000065478
NCBI RefSeq
NM_001267621
Target Region
Exon 4~5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Growth factor independence 1 (GFI1) is a DNA-binding zinc finger protein that acts as a transcriptional repressor [1,3,5]. It plays crucial roles in hematopoiesis, regulating the function of hematopoietic stem-and precursor cells, as well as differentiation along myeloid and lymphoid lineages [1]. GFI1 mediates transcriptional repression mainly by recruiting histone-modifying enzymes to its target genes, thus participating in epigenetic regulation [1].
In acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS), GFI1 has been implicated in pathogenesis. For instance, its expression levels correlate with patient survival and treatment response in both diseases, and can influence disease progression in experimental animal models [1]. A non-synonymous single nucleotide polymorphism (SNP), GFI1-36N, is a prognostic factor for AML and MDS development. This variant leads to genome-wide epigenetic changes at GFI1-occupied target gene promoters and enhancers, affecting the response of leukemic cells to epigenetic drugs [1]. In addition, GFI1-36N expression is associated with increased chromosomal aberrations, mutational burden, and impaired homologous recombination-directed DNA repair in leukemic cells. Targeting DNA repair-related proteins in GFI1-36N-expressing AML cells shows promise for treatment [4].
In the inner ear, Gfi1 is important for hair cell (HC) development, regulating HC-related gene expression. Ectopic expression of Gfi1 in supporting cells can potentially regenerate HCs [2].
In conclusion, GFI1 is a key regulator in hematopoiesis and inner ear development. Its role in diseases like AML and MDS is significant, especially in relation to epigenetic regulation and DNA repair. The study of GFI1, particularly through genetic models such as mouse models with GFI1-related mutations, provides valuable insights into its functions and potential therapeutic applications in hematological diseases and inner ear regeneration.
References:
1. Möröy, Tarik, Khandanpour, Cyrus. 2019. Role of GFI1 in Epigenetic Regulation of MDS and AML Pathogenesis: Mechanisms and Therapeutic Implications. In Frontiers in oncology, 9, 824. doi:10.3389/fonc.2019.00824. https://pubmed.ncbi.nlm.nih.gov/31508375/
2. Li, Zhuo, Chen, Hongzhi, Feng, Hao. 2025. Gfi1 in the inner ear: A retrospective review. In Developmental dynamics : an official publication of the American Association of Anatomists, , . doi:10.1002/dvdy.70019. https://pubmed.ncbi.nlm.nih.gov/40135914/
3. Beauchemin, Hugues, Möröy, Tarik. 2020. Multifaceted Actions of GFI1 and GFI1B in Hematopoietic Stem Cell Self-Renewal and Lineage Commitment. In Frontiers in genetics, 11, 591099. doi:10.3389/fgene.2020.591099. https://pubmed.ncbi.nlm.nih.gov/33193732/
4. Frank, Daria, Patnana, Pradeep Kumar, Vorwerk, Jan, Jayavelu, Ashok Kumar, Khandanpour, Cyrus. . Germ line variant GFI1-36N affects DNA repair and sensitizes AML cells to DNA damage and repair therapy. In Blood, 142, 2175-2191. doi:10.1182/blood.2022015752. https://pubmed.ncbi.nlm.nih.gov/37756525/
5. van der Meer, L T, Jansen, J H, van der Reijden, B A. 2010. Gfi1 and Gfi1b: key regulators of hematopoiesis. In Leukemia, 24, 1834-43. doi:10.1038/leu.2010.195. https://pubmed.ncbi.nlm.nih.gov/20861919/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen