C57BL/6JCya-Hnrnpkem1flox/Cya
Common Name:
Hnrnpk-flox
Product ID:
S-CKO-02926
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hnrnpk-flox
Strain ID
CKOCMP-15387-Hnrnpk-B6J-VA
Gene Name
Product ID
S-CKO-02926
Gene Alias
Hnrpk; KBBP; NOVA
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hnrnpkem1flox/Cya mice (Catalog S-CKO-02926) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000116403
NCBI RefSeq
NM_001301341.1
Target Region
Exon 6~7
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Hnrnpk, or heterogeneous nuclear ribonucleoprotein K, is a DNA/RNA-binding protein containing 3 K-homologous (KH) domains. It functions as an enhancer or repressor of gene transcription, and phosphorylation can alter its regulatory function, allowing it to serve as a docking platform for signal transduction proteins. Hnrnpk is involved in many cellular events, such as long non-coding RNA (lncRNA) regulation, cancer development, and bone homeostasis [1].
Deletion of Hnrnpk in mice accelerates the development of post-traumatic and age-dependent osteoarthritis (OA) by increasing WWC1 expression and activating the Hippo signaling pathway, indicating its role in maintaining articular cartilage homeostasis [2]. In hepatic progenitor cells, TNFR2-hnRNPK-YAP signaling axis is essential for primary liver cancer development, with hnRNPK acting downstream of TNFα-TNFR2 to interact with and stabilize YAP on target gene promoters [3]. Ablating Hnrnpk in mice causes dwarfism due to damaged survival and premature differentiation of growth plate chondrocytes, as it regulates the Hif1α-glycolysis axis by binding to Hif1a mRNA and promoting its degradation [4].
In summary, Hnrnpk is crucial for maintaining cellular and tissue homeostasis. Gene knockout mouse models have revealed its important roles in OA, primary liver cancer, and skeletal development. Understanding Hnrnpk's functions can provide insights into the mechanisms of these diseases and potential therapeutic targets.
References:
1. Wang, Ziyi, Qiu, Heng, He, Jianbo, Tickner, Jennifer, Xu, Jiake. 2019. The emerging roles of hnRNPK. In Journal of cellular physiology, 235, 1995-2008. doi:10.1002/jcp.29186. https://pubmed.ncbi.nlm.nih.gov/31538344/
2. Zhang, Shun, Zhang, Baolin, Liao, Zhiheng, Xu, Caixia, Su, Peiqiang. 2024. Hnrnpk protects against osteoarthritis through targeting WWC1 mRNA and inhibiting Hippo signaling pathway. In Molecular therapy : the journal of the American Society of Gene Therapy, 32, 1461-1478. doi:10.1016/j.ymthe.2024.02.027. https://pubmed.ncbi.nlm.nih.gov/38414246/
3. Meng, Yan, Zhao, Qiudong, An, Liwei, Zhou, Zhaocai, Wei, Lixin. 2021. A TNFR2-hnRNPK Axis Promotes Primary Liver Cancer Development via Activation of YAP Signaling in Hepatic Progenitor Cells. In Cancer research, 81, 3036-3050. doi:10.1158/0008-5472.CAN-20-3175. https://pubmed.ncbi.nlm.nih.gov/33619115/
4. Chen, Yuyu, Wu, Jinna, Zhang, Shun, Su, Peiqiang, Xu, Caixia. 2022. Hnrnpk maintains chondrocytes survival and function during growth plate development via regulating Hif1α-glycolysis axis. In Cell death & disease, 13, 803. doi:10.1038/s41419-022-05239-0. https://pubmed.ncbi.nlm.nih.gov/36127325/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen