C57BL/6JCya-Anxa1em1flox/Cya
Common Name:
Anxa1-flox
Product ID:
S-CKO-03421
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Anxa1-flox
Strain ID
CKOCMP-16952-Anxa1-B6J-VA
Gene Name
Product ID
S-CKO-03421
Gene Alias
Anx-1; Anx-A1; C430014K04Rik; Lpc-1; Lpc1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Anxa1em1flox/Cya mice (Catalog S-CKO-03421) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025561
NCBI RefSeq
NM_010730
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Anxa1, also known as Annexin-A1, is a protein-coding gene. It is involved in multiple biological processes such as immune regulation, cell phenotype switching, and cholesterol transport [1,2,3]. In the immune system, it can regulate microglial activation. It also plays a role in pathways like the NF-κB pathway [1].
In an acute aortic dissection (AAD) murine model, systemic deficiency of Anxa1 markedly progressed AAD, indicating its role in preventing AAD by inhibiting vascular smooth muscle cell (VSMC) phenotype switching. Anxa1 deficiency in VSMCs triggered the synthetic phenotype via down-regulation of the JunB/MYL9 pathway, leading to elevated inflammation and enhanced matrix metalloproteinases production [2]. In microglia, administration of Tat-NTS peptide led to a shift of Anxa1 subcellular localization, an increase in Anxa1 SUMOylation, and a transformation of microglia towards an anti-inflammatory phenotype, reducing the activation of the NF-κB pathway and subsequent release of pro-inflammatory factors, thus protecting neurons against cerebral ischemia-reperfusion injury [1].
In conclusion, Anxa1 is crucial in immune regulation, especially in microglial activation related to cerebral ischemia-reperfusion injury, and in preventing AAD through its effect on VSMC phenotype switching. Gene knockout or conditional knockout mouse models have been instrumental in revealing these functions, providing potential therapeutic targets for these disease areas.
References:
1. Zhou, Huijuan, Yan, Lulu, Huang, Hezhou, Sun, Ning, Shi, Jing. 2023. Tat-NTS peptide protects neurons against cerebral ischemia-reperfusion injury via ANXA1 SUMOylation in microglia. In Theranostics, 13, 5561-5583. doi:10.7150/thno.85390. https://pubmed.ncbi.nlm.nih.gov/37908731/
2. Zhou, Changping, Lin, Zhiyong, Cao, Huanhuan, Pan, Bing, Zheng, Lemin. . Anxa1 in smooth muscle cells protects against acute aortic dissection. In Cardiovascular research, 118, 1564-1582. doi:10.1093/cvr/cvab109. https://pubmed.ncbi.nlm.nih.gov/33757117/
3. Shen, Xin, Zhang, Shun, Guo, Zhu, Xing, Dongming, Chen, Wujun. 2020. The crosstalk of ABCA1 and ANXA1: a potential mechanism for protection against atherosclerosis. In Molecular medicine (Cambridge, Mass.), 26, 84. doi:10.1186/s10020-020-00213-y. https://pubmed.ncbi.nlm.nih.gov/32894039/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen