C57BL/6JCya-Tmed1em1flox/Cya
Common Name:
Tmed1-flox
Product ID:
S-CKO-03534
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmed1-flox
Strain ID
CKOCMP-17083-Tmed1-B6J-VA
Gene Name
Product ID
S-CKO-03534
Gene Alias
Il1rl1l; Ly84l; St2l
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmed1em1flox/Cya mice (Catalog S-CKO-03534) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034698
NCBI RefSeq
NM_010744
Target Region
Exon 2~4
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
TMED1, also known as p24γ1, is a member of the transmembrane emp24 domain-containing (TMED) protein family. These proteins are crucial for intracellular protein transport, participating in the bidirectional transport between the endoplasmic reticulum (ER) and the Golgi apparatus [1,3]. They are also involved in protein vesicular trafficking [2].
In C. elegans, tmed-1 (ortholog of human TMED1) belongs to the γ subfamily of TMED genes. tmed-1 and tmed-3 can compensate for each other, and their double mutants show defects in movement and vulva morphology, along with a delay in basement membrane breakdown during vulva development. This suggests a role for TMED1 in tissue reorganization during animal development [5]. In colorectal cancer (CRC), high expression of TMED1 is related to poor prognosis. Knocking down TMED1 reduces cell growth and increases apoptosis. Its expression is positively correlated with other TMED family members in CRC and is associated with immune cell infiltration and drug resistance [2]. Also, TMED1 associates with ST2L through its GOLD domain and the TIR domain of ST2L, and is required for optimal IL-33-induced IL-8 and IL-6 production, indicating its role in innate immune signaling [4].
In conclusion, TMED1 is essential for protein transport and vesicular trafficking. Studies in genetic models like C. elegans and in disease-relevant contexts such as CRC and innate immune signaling have revealed its roles in tissue development, cancer progression, and immune response regulation. These findings contribute to our understanding of normal biological processes and disease mechanisms, potentially paving the way for novel therapeutic strategies in related diseases.
References:
1. Mota, Danielly C A M, Cardoso, Iara A, Mori, Renan M, Costa-Filho, Antonio J, Mendes, Luis F S. 2021. Structural and thermodynamic analyses of human TMED1 (p24γ1) Golgi dynamics. In Biochimie, 192, 72-82. doi:10.1016/j.biochi.2021.10.002. https://pubmed.ncbi.nlm.nih.gov/34634369/
2. Guo, Xin, Zhou, Wei, Jin, Jinmei, Zhang, Lijun, Luan, Xin. 2024. Integrative Multi-Omics Analysis Identifies Transmembrane p24 Trafficking Protein 1 (TMED1) as a Potential Prognostic Marker in Colorectal Cancer. In Biology, 13, . doi:10.3390/biology13020083. https://pubmed.ncbi.nlm.nih.gov/38392302/
3. Zhou, Lv, Li, Huaixu, Yao, Hui, Gao, Peng, Cheng, Hongwei. 2023. TMED family genes and their roles in human diseases. In International journal of medical sciences, 20, 1732-1743. doi:10.7150/ijms.87272. https://pubmed.ncbi.nlm.nih.gov/37928880/
4. Connolly, Dympna J, O'Neill, Luke A J, McGettrick, Anne F. 2013. The GOLD domain-containing protein TMED1 is involved in interleukin-33 signaling. In The Journal of biological chemistry, 288, 5616-23. doi:10.1074/jbc.M112.403899. https://pubmed.ncbi.nlm.nih.gov/23319592/
5. Navarro, Kristen G, Chamberlin, Helen M. 2023. Genetic characterization of C. elegans TMED genes. In Developmental dynamics : an official publication of the American Association of Anatomists, 252, 1149-1161. doi:10.1002/dvdy.601. https://pubmed.ncbi.nlm.nih.gov/37204056/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen