C57BL/6NCya-Ptger4em1flox/Cya
Common Name
Ptger4-flox
Product ID
S-CKO-04539
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-19219-Ptger4-B6N-VA
Status
When using this mouse strain in a publication, please cite “Ptger4-flox Mouse (Catalog S-CKO-04539) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ptger4-flox
Strain ID
CKOCMP-19219-Ptger4-B6N-VA
Gene Name
Product ID
S-CKO-04539
Gene Alias
EP4, Ptgerep4
Background
C57BL/6NCya
Gene Full Name
prostaglandin E receptor 4 (subtype EP4)
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 15 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000047379
NCBI Transcript ID
NM_008965
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Overview of Gene Research
Ptger4, the prostaglandin E2 receptor 4, is crucial in maintaining gut homeostasis. It is involved in the prostaglandin E2 (PGE2) signaling pathway, where PGE2 produced by mesenchymal stromal cells can stimulate Ptger4 in epithelial cells [2]. Ptger4 has significance in immune regulation, bone metabolism, and is associated with various diseases [4]. Genetic models, especially knockout mouse models, have been pivotal in understanding its functions.
In a Csf1r-iCre Ptger4fl/fl mouse model of DSS-induced colitis, the absence of Ptger4 in macrophages led to defective mucosal healing and epithelial barrier regeneration. Mechanistically, PGE2 triggers CXCL1 secretion in monocyte-derived PTGER4+ macrophages via mitogen-activated protein kinases (MAPKs), and CXCL1 drives epithelial cell differentiation and proliferation during colitis [1]. In the context of intestinal tumorigenesis, epithelial-specific ablation of Ptger4 in mutant mice misdirected the regenerative reprogramming of stem cells, preventing Sca-1+ cell expansion and sporadic tumour initiation, demonstrating the role of PGE2-Ptger4 in controlling tumour-initiating stem cells [3].
In conclusion, Ptger4 plays essential roles in maintaining the integrity of the intestinal epithelium and in the regulation of intestinal tumorigenesis as revealed through gene-knockout mouse models. These findings highlight its potential as a therapeutic target, especially in diseases like inflammatory bowel disease and colorectal cancer.
References：
1. Na, Yi Rang, Jung, Daun, Stakenborg, Michelle, Matteoli, Gianluca, Seok, Seung Hyeok. 2021. Prostaglandin E2 receptor PTGER4-expressing macrophages promote intestinal epithelial barrier regeneration upon inflammation. In Gut, 70, 2249-2260. doi:10.1136/gutjnl-2020-322146. https://pubmed.ncbi.nlm.nih.gov/33558271/
2. Anbazhagan, Murugadas, Sharma, Garima, Murthy, Shanta, Kugathasan, Subra, Matthews, Jason D. 2024. PTGER4 signaling regulates class IIa HDAC function and SPINK4 mRNA levels in rectal epithelial cells. In Cell communication and signaling : CCS, 22, 493. doi:10.1186/s12964-024-01879-1. https://pubmed.ncbi.nlm.nih.gov/39396982/
3. Roulis, Manolis, Kaklamanos, Aimilios, Schernthanner, Marina, Kollias, George, Flavell, Richard A. 2020. Paracrine orchestration of intestinal tumorigenesis by a mesenchymal niche. In Nature, 580, 524-529. doi:10.1038/s41586-020-2166-3. https://pubmed.ncbi.nlm.nih.gov/32322056/
4. Rodriguez-Rodriguez, Luis, Ivorra-Cortes, Jose, Carmona, F David, González-Álvaro, Isidoro, Fernandez-Gutiérrez, Benjamín. 2015. PTGER4 gene variant rs76523431 is a candidate risk factor for radiological joint damage in rheumatoid arthritis patients: a genetic study of six cohorts. In Arthritis research & therapy, 17, 306. doi:10.1186/s13075-015-0830-z. https://pubmed.ncbi.nlm.nih.gov/26538147/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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