C57BL/6JCya-Rangap1em1flox/Cya
Common Name:
Rangap1-flox
Product ID:
S-CKO-04694
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rangap1-flox
Strain ID
CKOCMP-19387-Rangap1-B6J-VA
Gene Name
Product ID
S-CKO-04694
Gene Alias
Fug1; mKIAA1835
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rangap1em1flox/Cya mice (Catalog S-CKO-04694) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052374
NCBI RefSeq
NM_001146174.1
Target Region
Exon 3~4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
RanGAP1, short for Ran GTPase-activating protein 1, is crucial for maintaining chromosome stability and is involved in multiple cellular processes. It participates in the spindle-assembly checkpoint (SAC) regulation and chromatid decatenation during mitosis, safeguarding mitotic chromosome integrity. It also has a role in nuclear protein export through its interaction with RAS•GTP [3,4,5].
In gene knockout studies, RanGAP1 deficiency in mouse osteoprogenitors leads to chromothripsis, inactivation of Rb1, degradation of p53, DNA damage repair failure, and rapid osteosarcoma tumorigenesis. In limb and head mesenchymal progenitor cells expressing Prx1 in mice, RanGAP1 ablation causes embryonic lethality, severe cartilage and bone dysplasia, and chromosome missegregation [1,2].
In conclusion, RanGAP1 is essential for maintaining chromosome stability during cell division and bone development. Its deficiency in KO mouse models reveals its role in promoting tumorigenesis in osteosarcoma and disrupting skeletal development. Understanding RanGAP1 provides insights into the mechanisms of chromosome stability and the development of related diseases.
References：
1. Gong, Yan, Zou, Shitian, Deng, Daizhao, Zou, Zhipeng, Bai, Xiaochun. 2023. Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma. In Developmental cell, 58, 192-210.e11. doi:10.1016/j.devcel.2022.12.012. https://pubmed.ncbi.nlm.nih.gov/36696903/
2. Huang, Minjun, Chen, Bochong, Chen, Xiaoli, Bai, Xiaochun, Gong, Yan. 2024. RanGAP1 maintains chromosome stability in limb bud mesenchymal cells during bone development. In Cellular signalling, 120, 111222. doi:10.1016/j.cellsig.2024.111222. https://pubmed.ncbi.nlm.nih.gov/38729327/
3. Tripathi, Brajendra K, Hirsh, Nicole H, Qian, Xiaolan, Walters, Kylie J, Lowy, Douglas R. 2024. The pro-oncogenic noncanonical activity of a RAS•GTP:RanGAP1 complex facilitates nuclear protein export. In Nature cancer, 5, 1902-1918. doi:10.1038/s43018-024-00847-5. https://pubmed.ncbi.nlm.nih.gov/39528835/
4. Joseph, Jomon, Liu, Song-Tao, Jablonski, Sandra A, Yen, Tim J, Dasso, Mary. . The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo. In Current biology : CB, 14, 611-7. doi:. https://pubmed.ncbi.nlm.nih.gov/15062103/
5. Cha, Keith, Sen, Progga, Raghunayakula, Sarita, Zhang, Xiang-Dong. 2015. The Cellular Distribution of RanGAP1 Is Regulated by CRM1-Mediated Nuclear Export in Mammalian Cells. In PloS one, 10, e0141309. doi:10.1371/journal.pone.0141309. https://pubmed.ncbi.nlm.nih.gov/26506250/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen