C57BL/6JCya-Tmprss11aem1flox/Cya
Common Name:
Tmprss11a-flox
Product ID:
S-CKO-04717
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tmprss11a-flox
Strain ID
CKOCMP-194597-Tmprss11a-B6J-VA
Gene Name
Product ID
S-CKO-04717
Gene Alias
Gm7
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmprss11aem1flox/Cya mice (Catalog S-CKO-04717) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000101073
NCBI RefSeq
NM_001033233
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Tmprss11a, a member of the type II transmembrane serine protease (TTSP) family, has been implicated in various biological processes. It is involved in viral infection as it can cleave and activate spike proteins of coronaviruses such as SARS and MERS, as well as the influenza A virus hemagglutinin [2,3]. In addition, it may have a role in cell migration and wound healing, and is associated with cellular senescence [1].
Studies on Tmprss11a have provided insights into its functions. In skin, its levels increase with age, and overexpression decreases cell migration and spreading while promoting cellular senescence. ShRNA-mediated downregulation of Tmprss11a improves wound healing in the skin of old mice, indicating its tissue-specific role in wound repair [1]. Tmprss11a activates viral proteins intracellularly through autocatalysis, which is different from the extracellular activation mechanism of other TTSPs [2]. It also exhibits differential sensitivity to blockade by cellular serine protease inhibitors compared to related enzymes [3].
In conclusion, Tmprss11a is a multifunctional gene involved in viral infection, cell migration, wound healing, and cellular senescence. Research on Tmprss11a, including the use of techniques like shRNA-mediated downregulation (a form of functional study similar to gene knockout-like effects), has provided valuable insights into its role in these biological processes and potential implications for diseases related to viral infections and wound healing [1,2,3].
References:
1. Fernandez, Christian, Burgos, Andres, Morales, Diego, Conboy, Irina M, Cáceres, Mónica. . TMPRSS11a is a novel age-altered, tissue specific regulator of migration and wound healing. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35, e21597. doi:10.1096/fj.202002253RRR. https://pubmed.ncbi.nlm.nih.gov/33908663/
2. Zhang, Ce, Zhang, Yikai, Zhang, Shengnan, Dong, Ningzheng, Wu, Qingyu. 2020. Intracellular autoactivation of TMPRSS11A, an airway epithelial transmembrane serine protease. In The Journal of biological chemistry, 295, 12686-12696. doi:10.1074/jbc.RA120.014525. https://pubmed.ncbi.nlm.nih.gov/32675285/
3. Zmora, Pawel, Hoffmann, Markus, Kollmus, Heike, Schughart, Klaus, Pöhlmann, Stefan. 2018. TMPRSS11A activates the influenza A virus hemagglutinin and the MERS coronavirus spike protein and is insensitive against blockade by HAI-1. In The Journal of biological chemistry, 293, 13863-13873. doi:10.1074/jbc.RA118.001273. https://pubmed.ncbi.nlm.nih.gov/29976755/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen