C57BL/6JCya-Rhagem1flox/Cya
Common Name:
Rhag-flox
Product ID:
S-CKO-04798
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rhag-flox
Strain ID
CKOCMP-19743-Rhag-B6J-VA
Gene Name
Product ID
S-CKO-04798
Gene Alias
CD241; Rh50; Rh50A
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rhagem1flox/Cya mice (Catalog S-CKO-04798) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024721
NCBI RefSeq
NM_011269
Target Region
Exon 2~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Rhag, the gene encoding Rh-associated glycoprotein, is crucial for maintaining the structure and stability of erythrocytes. It is essential for the assembly of the Rh protein complex in the erythrocyte membrane and for the expression of Rh antigens [2,3]. Rhag also contributes significantly to the NH₃ conductance of erythrocytes [1].
Mice with inactivated Rhag genes (Rhag⁻/⁻) have been generated [4]. Red cells from these KO mice totally lack Rhag and Rh proteins. Such KO mouse models help in delineating the antigenic, biochemical, and functional abnormalities of red cells, as well as the impact on hematological parameters and erythropoiesis [4]. In addition, missense mutations in the RhAG gene in humans can lead to spherocytosis and deficiencies in Rh complex formation [2].
In conclusion, Rhag plays a vital role in maintaining erythrocyte structure and stability, and in Rh antigen expression and NH₃ conductance. The study of Rhag KO mouse models has been valuable in understanding its function and how its absence affects red cell properties and hematopoiesis, with implications for conditions like spherocytosis.
References：
1. Ripoche, P, Goossens, D, Devuyst, O, Verkman, A S, Cartron, J-P. 2006. Role of RhAG and AQP1 in NH3 and CO2 gas transport in red cell ghosts: a stopped-flow analysis. In Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine, 13, 117-22. doi:. https://pubmed.ncbi.nlm.nih.gov/16574458/
2. Mu, S, Cui, Y, Wang, W, Zhu, O, Zhu, D. 2018. A RHAG point mutation selectively disrupts Rh antigen expression. In Transfusion medicine (Oxford, England), 29, 121-127. doi:10.1111/tme.12519. https://pubmed.ncbi.nlm.nih.gov/29508504/
3. Avent, N D, Reid, M E. . The Rh blood group system: a review. In Blood, 95, 375-87. doi:. https://pubmed.ncbi.nlm.nih.gov/10627438/
4. Goossens, D, Bony, V, Gane, P, Colin, Y, Cartron, J-P. 2006. Generation of mice with inactivated Rh or Rhag genes. In Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine, 13, 164-6. doi:. https://pubmed.ncbi.nlm.nih.gov/16581281/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen