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C57BL/6JCya-Med22em1flox/Cya
Common Name:
Med22-flox
Product ID:
S-CKO-05441
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Med22-flox
Strain ID
CKOCMP-20933-Med22-B6J-VA
Gene Name
Med22
Product ID
S-CKO-05441
Gene Alias
Surf-5; Surf5
Background
C57BL/6JCya
NCBI ID
20933
Modification
Conditional knockout
Chromosome
2
Phenotype
MGI:98446
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Med22em1flox/Cya mice (Catalog S-CKO-05441) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015920
NCBI RefSeq
NM_001033908
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Med22, a subunit of the Mediator complex, is crucial for gene transcription as the Mediator connects transcriptional regulators and RNA polymerase II [4]. The Mediator head module, which includes Med22, is involved in the recruitment of TFIIH and TFIIE to the preinitiation complex, playing a role in the regulation of gene expression [6].

Podocyte-specific Med22 knockout mice revealed that while Med22 is not essential for normal podocyte maturation, it is critical for maintaining podocyte health. Its deficiency leads to intracellular vacuole formation, podocyte loss, progressive glomerular disease, and ultimately death due to renal failure, suggesting a role in the development of glomerulopathies [1].

In patients with major depressive disorder, genetic and environmental components of MED22 expression regulation are associated with childhood trauma, and its genetic variations are linked to higher neuroticism in healthy volunteers. Moreover, trauma-focused psychotherapy decreases MED22 expression, and baseline levels are associated with relapse, indicating its potential role in detecting relapse [2].

In bladder cancer, MED22 was identified as a potential new pharmaceutical target when analyzing THZ1-sensitive and super-enhancer-associated oncogenes [3].

In Drosophila spermatocytes, loss of Med22 function causes meiosis I maturation arrest and male infertility, similar to the loss of function of other related complexes, suggesting its role in the regulation of genes required for spermatid differentiation [5].

In summary, Med22 plays essential roles in maintaining podocyte health, is associated with major depressive disorder, and may serve as a potential target in bladder cancer. In Drosophila, it is crucial for spermatid differentiation. Gene-knockout models, especially in mice, have been instrumental in uncovering these functions, providing valuable insights into the biological processes and disease conditions related to Med22.

References:
1. Rodriguez, Patricia Q, Unnersjö-Jess, David, Zambrano, Sonia S, Ebarasi, Lwaki, Patrakka, Jaakko. 2020. Inactivation of mediator complex protein 22 in podocytes results in intracellular vacuole formation, podocyte loss and premature death. In Scientific reports, 10, 20037. doi:10.1038/s41598-020-76870-0. https://pubmed.ncbi.nlm.nih.gov/33208756/
2. Carvalho Silva, Rosana, Maffioletti, Elisabetta, Magri, Chiara, Gennarelli, Massimo, Minelli, Alessandra. 2025. The role of MED22 and its transcriptional interactions with childhood trauma and trauma-focused psychotherapy in patients with major depressive disorder. In Biological psychology, 197, 109039. doi:10.1016/j.biopsycho.2025.109039. https://pubmed.ncbi.nlm.nih.gov/40250788/
3. Yang, Yafei, Jiang, Donggen, Zhou, Ziyu, Wu, Song, Pang, Jun. 2021. CDK7 blockade suppresses super-enhancer-associated oncogenes in bladder cancer. In Cellular oncology (Dordrecht, Netherlands), 44, 871-887. doi:10.1007/s13402-021-00608-x. https://pubmed.ncbi.nlm.nih.gov/33905040/
4. Larivière, Laurent, Plaschka, Clemens, Seizl, Martin, Kurth, Fabian, Cramer, Patrick. 2012. Structure of the Mediator head module. In Nature, 492, 448-51. doi:10.1038/nature11670. https://pubmed.ncbi.nlm.nih.gov/23123849/
5. Lu, Chenggang, Fuller, Margaret T. 2015. Recruitment of Mediator Complex by Cell Type and Stage-Specific Factors Required for Tissue-Specific TAF Dependent Gene Activation in an Adult Stem Cell Lineage. In PLoS genetics, 11, e1005701. doi:10.1371/journal.pgen.1005701. https://pubmed.ncbi.nlm.nih.gov/26624996/
6. Esnault, Cyril, Ghavi-Helm, Yad, Brun, Sylvain, Holstege, Frank, Werner, Michel. . Mediator-dependent recruitment of TFIIH modules in preinitiation complex. In Molecular cell, 31, 337-46. doi:10.1016/j.molcel.2008.06.021. https://pubmed.ncbi.nlm.nih.gov/18691966/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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