C57BL/6JCya-Nlrp3em1flox/Cya
Common Name:
Nlrp3-flox
Product ID:
S-CKO-06042
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nlrp3-flox
Strain ID
CKOCMP-216799-Nlrp3-B6J-VA
Gene Name
Product ID
S-CKO-06042
Gene Alias
AGTAVPRL; AII/AVP; Cias1; FCAS; FCU; MWS; Mmig1; NALP3; Pypaf1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nlrp3em1flox/Cya mice (Catalog S-CKO-06042) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000101148
NCBI RefSeq
NM_145827
Target Region
Exon 4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Nlrp3, also known as NACHT, leucine-rich repeat (LRR), and pyrin domain (PYD)-containing protein 3, is a key component of the NLRP3 inflammasome. The NLRP3 inflammasome is a multi-protein signaling complex that triggers the activation of inflammatory caspases and the maturation of interleukin-1β. It is involved in the body's inflammatory response to various "danger" situations such as infection and metabolic dysregulation, and has been linked to numerous autoinflammatory and autoimmune diseases [2].
Mitophagy/autophagy blockade leads to the accumulation of damaged, ROS-generating mitochondria, which in turn activates the NLRP3 inflammasome. Inhibition of the voltage-dependent anion channel, which dysregulates mitochondrial activity, suppresses both ROS generation and inflammasome activation, indicating that NLRP3 inflammasome senses mitochondrial dysfunction [1]. Different NLRP3 stimuli cause the disassembly of the trans-Golgi network (TGN). NLRP3 is recruited to the dispersed TGN through an ionic bond with phosphatidylinositol-4-phosphate (PtdIns4P), leading to its aggregation and activation [3]. Palmitoylation of NLRP3 Cys126 by ZDHHC7 promotes NLRP3-mediated inflammasome activation, and perturbation of this palmitoylation diminishes NLRP3 activation in macrophages and in vivo [4].
In conclusion, NLRP3 plays a crucial role in the inflammatory response by sensing mitochondrial and organelle dysfunction. Its activation is regulated by multiple mechanisms related to organelles and post-translational modifications. Understanding NLRP3 function through model-based research, especially in relation to its role in various diseases such as cancer, liver injury, cardiovascular diseases, and COVID-19, may offer potential therapeutic strategies for these conditions [5,6,7,8].
References:
1. Zhou, Rongbin, Yazdi, Amir S, Menu, Philippe, Tschopp, Jürg. 2010. A role for mitochondria in NLRP3 inflammasome activation. In Nature, 469, 221-5. doi:10.1038/nature09663. https://pubmed.ncbi.nlm.nih.gov/21124315/
2. Jo, Eun-Kyeong, Kim, Jin Kyung, Shin, Dong-Min, Sasakawa, Chihiro. 2015. Molecular mechanisms regulating NLRP3 inflammasome activation. In Cellular & molecular immunology, 13, 148-59. doi:10.1038/cmi.2015.95. https://pubmed.ncbi.nlm.nih.gov/26549800/
3. Chen, Jueqi, Chen, Zhijian J. 2018. PtdIns4P on dispersed trans-Golgi network mediates NLRP3 inflammasome activation. In Nature, 564, 71-76. doi:10.1038/s41586-018-0761-3. https://pubmed.ncbi.nlm.nih.gov/30487600/
4. Yu, Tao, Hou, Dan, Zhao, Jiaqi, Linder, Maurine E, Lin, Hening. 2024. NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation. In Cell reports, 43, 114070. doi:10.1016/j.celrep.2024.114070. https://pubmed.ncbi.nlm.nih.gov/38583156/
5. Tengesdal, Isak W, Dinarello, Charles A, Marchetti, Carlo. 2023. NLRP3 and cancer: Pathogenesis and therapeutic opportunities. In Pharmacology & therapeutics, 251, 108545. doi:10.1016/j.pharmthera.2023.108545. https://pubmed.ncbi.nlm.nih.gov/37866732/
6. Yu, Chaoqun, Chen, Peng, Miao, Longyu, Di, Guohu. 2023. The Role of the NLRP3 Inflammasome and Programmed Cell Death in Acute Liver Injury. In International journal of molecular sciences, 24, . doi:10.3390/ijms24043067. https://pubmed.ncbi.nlm.nih.gov/36834481/
7. Mezzaroma, Eleonora, Abbate, Antonio, Toldo, Stefano. 2021. NLRP3 Inflammasome Inhibitors in Cardiovascular Diseases. In Molecules (Basel, Switzerland), 26, . doi:10.3390/molecules26040976. https://pubmed.ncbi.nlm.nih.gov/33673188/
8. Yin, Maureen, Marrone, Laura, Peace, Christian G, O'Neill, Luke A J. . NLRP3, the inflammasome and COVID-19 infection. In QJM : monthly journal of the Association of Physicians, 116, 502-507. doi:10.1093/qjmed/hcad011. https://pubmed.ncbi.nlm.nih.gov/36661317/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen