C57BL/6JCya-Tob1em1flox/Cya
Common Name:
Tob1-flox
Product ID:
S-CKO-06448
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tob1-flox
Strain ID
CKOCMP-22057-Tob1-B6J-VA
Gene Name
Product ID
S-CKO-06448
Gene Alias
Tob; Trob
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tob1em1flox/Cya mice (Catalog S-CKO-06448) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041589
NCBI RefSeq
NM_009427
Target Region
Exon 2
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
TOB1, also known as Transducer of ERBB2.1, is a tumor-suppressor protein. It negatively regulates the receptor tyrosine-kinase ERBB2. TOB1 is involved in multiple biological processes such as cell proliferation, autophagy, and immune cell regulation. It participates in pathways like the AKT/mTOR signaling pathway and is associated with the regulation of cyclin D1, cyclin-dependent kinase expressions, and CDK inhibitors [6].
In various diseases, TOB1 shows diverse functions. In gastric cancer, it can suppress the malignant phenotype of cancer cells. High levels of cTOB1/nTOB1+CD66b+ neutrophils are linked to a better prognosis, and TOB1-transfected neutrophils can affect the apoptosis and malignant phenotype of gastric cancer cells [1]. In pancreatic cancer, TOB1 is downregulated, and its overexpression reduces the proliferation of K-Ras wild-type pancreatic cancer cells [3]. In muscle, loss of Tob1 promotes muscle regeneration through muscle stem cell expansion [4]. In IBD, TOB1 restrains intestinal mucosal inflammation by suppressing Th1/Th17 cell-mediated immune responses via the Smad4/5-ID2 pathway [5]. In FMDV infection, TOB1-knockout significantly inhibits FMDV infection both in vitro and in vivo, by positively regulating the expression of RIG-I and MDA5 and inhibiting FMDV adsorption and internalization mediated by the EGFR/ERBB2 pathway [2].
In conclusion, TOB1 is a multifunctional protein with a crucial role in tumor suppression, immune regulation, and tissue regeneration. Gene knockout models, especially in mice, have been instrumental in revealing its functions in various disease conditions such as cancer, viral infection, and inflammatory bowel disease. These studies help in understanding the underlying mechanisms and potentially developing new therapeutic strategies.
References:
1. Zhang, Jinfeng, Li, Yunlong, Chen, Jing, Fu, Songbin, Yu, Jingcui. 2024. TOB1 modulates neutrophil phenotypes to influence gastric cancer progression and immunotherapy efficacy. In Frontiers in immunology, 15, 1369087. doi:10.3389/fimmu.2024.1369087. https://pubmed.ncbi.nlm.nih.gov/38617839/
2. Peng, Gaochuang, Liu, Tianran, Qi, Xiaolan, Li, Dan, Zheng, Haixue. 2024. A genome-wide CRISPR screening uncovers that TOB1 acts as a key host factor for FMDV infection via both IFN and EGFR mediated pathways. In PLoS pathogens, 20, e1012104. doi:10.1371/journal.ppat.1012104. https://pubmed.ncbi.nlm.nih.gov/38512977/
3. Bai, Yuru, Qiao, Lu, Xie, Ning, Wang, Jinhai, Liu, Na. 2019. TOB1 suppresses proliferation in K-Ras wild-type pancreatic cancer. In Cancer medicine, 9, 1503-1514. doi:10.1002/cam4.2756. https://pubmed.ncbi.nlm.nih.gov/31891232/
4. Kitajima, Yasuo, Yoshioka, Kiyoshi, Mikumo, Yoko, Ohkawa, Yasuyuki, Ono, Yusuke. 2024. Loss of Tob1 promotes muscle regeneration through muscle stem cell expansion. In Journal of cell science, 137, . doi:10.1242/jcs.261886. https://pubmed.ncbi.nlm.nih.gov/39037211/
5. Lin, Ritian, Ma, Caiyun, Fang, Leilei, Cong, Yingzi, Liu, Zhanju. 2021. TOB1 Blocks Intestinal Mucosal Inflammation Through Inducing ID2-Mediated Suppression of Th1/Th17 Cell Immune Responses in IBD. In Cellular and molecular gastroenterology and hepatology, 13, 1201-1221. doi:10.1016/j.jcmgh.2021.12.007. https://pubmed.ncbi.nlm.nih.gov/34920145/
6. Lee, Hun Seok, Kundu, Juthika, Kim, Ryong Nam, Shin, Young Kee. 2015. Transducer of ERBB2.1 (TOB1) as a Tumor Suppressor: A Mechanistic Perspective. In International journal of molecular sciences, 16, 29815-28. doi:10.3390/ijms161226203. https://pubmed.ncbi.nlm.nih.gov/26694352/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen